Bismark report for: ../../data/03-Peve-bismark/POR-262-TP4_R1_001.fastp-trim.fq.gz and ../../data/03-Peve-bismark/POR-262-TP4_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/srlab/sr320/github/timeseries_molecular/E-Peve/data/ with the specified options: -q --score-min L,0,-0.6 -p 12 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 47364179 Number of paired-end alignments with a unique best hit: 29325159 Mapping efficiency: 61.9% Sequence pairs with no alignments under any condition: 13996238 Sequence pairs did not map uniquely: 4042782 Sequence pairs which were discarded because genomic sequence could not be extracted: 4976 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 14678288 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 14641895 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1455052913 Total methylated C's in CpG context: 20194055 Total methylated C's in CHG context: 1363295 Total methylated C's in CHH context: 5425580 Total methylated C's in Unknown context: 32307 Total unmethylated C's in CpG context: 208900157 Total unmethylated C's in CHG context: 254356912 Total unmethylated C's in CHH context: 964812914 Total unmethylated C's in Unknown context: 1874194 C methylated in CpG context: 8.8% C methylated in CHG context: 0.5% C methylated in CHH context: 0.6% C methylated in Unknown context (CN or CHN): 1.7% Bismark completed in 0d 3h 25m 23s