Bismark report for: ../../data/03-Peve-bismark/POR-260-TP1_R1_001.fastp-trim.fq.gz and ../../data/03-Peve-bismark/POR-260-TP1_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/srlab/sr320/github/timeseries_molecular/E-Peve/data/ with the specified options: -q --score-min L,0,-0.6 -p 12 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 36459375 Number of paired-end alignments with a unique best hit: 24715261 Mapping efficiency: 67.8% Sequence pairs with no alignments under any condition: 8505541 Sequence pairs did not map uniquely: 3238573 Sequence pairs which were discarded because genomic sequence could not be extracted: 4215 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 12370171 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 12340875 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1257683871 Total methylated C's in CpG context: 18095565 Total methylated C's in CHG context: 1028039 Total methylated C's in CHH context: 4029935 Total methylated C's in Unknown context: 25794 Total unmethylated C's in CpG context: 179517759 Total unmethylated C's in CHG context: 219716725 Total unmethylated C's in CHH context: 835295848 Total unmethylated C's in Unknown context: 1619439 C methylated in CpG context: 9.2% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in Unknown context (CN or CHN): 1.6% Bismark completed in 0d 2h 45m 1s