Bismark report for: ../../data/03-Peve-bismark/POR-216-TP1_R1_001.fastp-trim.fq.gz and ../../data/03-Peve-bismark/POR-216-TP1_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/srlab/sr320/github/timeseries_molecular/E-Peve/data/ with the specified options: -q --score-min L,0,-0.6 -p 12 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 34731826 Number of paired-end alignments with a unique best hit: 22092659 Mapping efficiency: 63.6% Sequence pairs with no alignments under any condition: 9659123 Sequence pairs did not map uniquely: 2980044 Sequence pairs which were discarded because genomic sequence could not be extracted: 3733 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11054556 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 11034370 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1127328046 Total methylated C's in CpG context: 13873180 Total methylated C's in CHG context: 1136402 Total methylated C's in CHH context: 4463628 Total methylated C's in Unknown context: 23413 Total unmethylated C's in CpG context: 169871416 Total unmethylated C's in CHG context: 197944708 Total unmethylated C's in CHH context: 740038712 Total unmethylated C's in Unknown context: 1429305 C methylated in CpG context: 7.6% C methylated in CHG context: 0.6% C methylated in CHH context: 0.6% C methylated in Unknown context (CN or CHN): 1.6% Bismark completed in 0d 2h 57m 54s