FastQCFastQC Report
Mon 24 Feb 2025
POR-83-TP3_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-83-TP3_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18612942
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC1598500.8588110358910482No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT1073470.576733113980584No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA1049070.5636239558474958No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA826430.4440082604888577No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT713900.3835503275086765No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG709920.3814120304033613No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG658350.3537055023327317No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT621660.3339934116809691No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC569690.30607197937864955No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG556910.2992057891761549No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA528560.2839744517551282No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT490320.26342960720556696No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT485890.2610495428396005No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA483640.25984070653634445No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA481930.2589219909458698No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA475120.25526324640134807No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC431910.2320482167730389No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG404570.21735951253702934No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG383760.20617911988335857No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA377090.20259559181992828No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA375540.20176283792212968No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA370920.1992806940461105No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC366900.19712090651762626No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT365970.1966212541789471No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC353950.19016338201666344No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG351270.18872352366434067No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC345390.18556443145849807No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC343010.18428575127994273No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT340730.1830607971593099No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG337010.18106218780459316No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTT335260.18012198179094954No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC332830.17881643858343296No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA332080.17841349314901428No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC325340.17479235684503824No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA321270.1726057062875928No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT316110.16983344169879216No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC313230.16828613123062436No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT310580.1668623906956783No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC307930.16543865016073223No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT299890.16111907510376383No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA295730.15888407109418812No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT295530.15877661897834314No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG293820.1578579033878685No Hit
TGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATG291030.15635894637183095No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG285910.15360817220619932No Hit
AAAATGGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATA284400.1527969087315697No Hit
TACTTATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTT280310.15059951296253973No Hit
CTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA279880.150368490913473No Hit
CAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAAC277060.1488534160800587No Hit
AATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT273510.1469461410238102No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC269140.14459831229259726No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG266740.14330888690245744No Hit
GAACTGGCAGTGACTAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT265310.14254060427416582No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA264600.1421591492629161No Hit
AAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGG263850.1417562038284974No Hit
TTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAGAAGCACT263140.1413747488172477No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG256040.1375601987047507No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC254640.1368080338938358No Hit
AAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAA252660.13574425794697043No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA252590.1357066497064247No Hit
GAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGTGGTA246410.13238637932681466No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA245130.13169868578540672No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT244720.13147840894792454No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA241660.12983439157549623No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG240420.12916818845725733No Hit
AGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTC240320.12911446239933483No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG233960.12569748511546427No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA233480.12543960003743632No Hit
TTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGT228960.12301118221933964No Hit
ACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAA225230.12100720025883065No Hit
TCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGA224660.12070096172867245No Hit
CATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGT221980.11926110337634964No Hit
CCATTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAA221770.1191482786547124No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG221550.1190300813272829No Hit
AAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAAGCCGGACGAC221140.1188098044898007No Hit
AAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTT218250.11725712141584066No Hit
TTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGG215120.11557549580286663No Hit
AAGGTAGGTCCGGACGCTCGCGTTCGGACTGTTACAGCCCGGTTCTCTCG214180.1150704708583952No Hit
TCAACTGTCGATGGTAAGGTAGTGGCTTACCATGGTTGCAACGGGTGACG213180.11453321027917027No Hit
TAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCG210760.11323303967744594No Hit
ACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTC210520.11310409713843196No Hit
GCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTA209010.11229283366380233No Hit
AATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTG208010.11175557308457737No Hit
AGACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTC207630.11155141406447192No Hit
GCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAG207030.11122905771693695No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTG206590.11099266306207797No Hit
GGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACG206240.11080462185934926No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG203890.10954205949817068No Hit
CCGGAGATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTC202180.10862334390769605No Hit
GGCTCGTAGCGATTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGG199710.10729631027701049No Hit
TTGAAGCCGGACGACGAATGAGAGTGCCTAGTGGGCCATTTTTGGTAAGC198090.10642594813866608No Hit
AGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGG195660.10512040493114952No Hit
GGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATG195380.10496997196896654No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA194410.10444882920711836No Hit
TTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCC193380.10389545081051668No Hit
GTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATG192250.10328834635599252No Hit
CTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAGTGGGAG190010.10208488265852868No Hit
GTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACGG189600.10186460582104645No Hit
AGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGT189120.10160672074301849No Hit
GTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTAT188330.1011822848854308No Hit
AACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGAC186850.10038713922817791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)