FastQCFastQC Report
Mon 24 Feb 2025
POR-245-TP4_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-245-TP4_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16925807
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC539310.3186317792705541No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT410700.2426472191252092No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA359830.21259252217634292No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG320870.1895744173379739No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT290280.17150142383166722No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA260080.15365884769925595No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT225750.13337621065867053No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC224260.13249589812763435No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT223830.1322418482025702No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG223560.1320823284821811No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC223280.1319169006239998No Hit
GCTAAATTGGACGGGAGATTCGAAAGAGCGAAGAAGAAGCTTTAAACTCG211640.1250398282338916No Hit
CGAAGAAGAAGCTTTAAACTCGTGTTTCAATGCGGAGACGCAATGAAGAG210450.12433675983662107No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA208110.12295425559324881No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG201580.11909624161494929No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA200060.11819820467053653No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA199720.11799732798560211No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG192160.11353077581470708No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC183390.10834933897095719No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA177610.10493443532707185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)