FastQCFastQC Report
Mon 24 Feb 2025
POR-245-TP2_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-245-TP2_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574371
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC1853441.1182566143837374No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT1070900.6461180336798301No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA978330.590266743757576No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG710120.4284446148816145No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA671460.40511944616178797No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG651060.3928112867752267No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT605390.3652566966191356No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG569570.3436450167550853No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT564920.340839480424325No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC525780.3172247079542264No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC509370.30732387974180136No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA505870.3052121857294011No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT469540.2832928018806868No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA409780.24723713497181884No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC404080.24379809043733847No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA383490.2313752962329611No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA381690.230289282169441No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA374290.2258245576860805No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG356270.21495235022795134No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG353580.21332936254413515No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA350570.21151330569347096No Hit
TGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATG338800.2044119804003422No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA337420.20357936961831008No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC334800.20199861581474193No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG334390.2017512459447179No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA325090.19614017328319727No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT318900.1924054915869809No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT310470.18731932572282833No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC294500.17768396761481928No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG293560.17711682693720324No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT289070.17440782518986692No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG286540.17288137208947477No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC284720.17178329120302666No Hit
AAAATGGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATA284630.17172899049985063No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA280310.16912255674740237No Hit
AATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT280120.16900792192958636No Hit
CTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA276160.1666186909898421No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC275840.16642562182299406No Hit
TACTTATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTT274370.16553871033778597No Hit
AAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGG274260.16547234281168197No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG267320.1612851552556655No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT266050.16051891199973742No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC263360.15889592431592126No Hit
AGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTC257950.1556318487138969No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA256570.1547992379318648No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC254350.15345982058685667No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT252020.1520540357157445No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT251790.1519152672520725No Hit
CAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAAC243420.14686530185670396No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG236470.14267208088922348No Hit
GAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGTGGTA235800.14226784232113546No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG233730.1410189261480873No Hit
ACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAA232960.14055435346535927No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG229140.13824959028611103No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC223650.13493724739237464No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA221130.13341682770344648No Hit
TTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCC218860.13204724330111833No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG218460.1318059068425583No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC218120.1316007708527823No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC216460.1305992245497582No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA216250.13047252290901418No Hit
GAACTGGCAGTGACTAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT214850.12962784530405408No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATT211140.12738944965090984No Hit
ACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTC210680.1271119127235658No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG209830.12659907274912574No Hit
TTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGT207120.12496401824238157No Hit
AAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAA206540.12461408037746952No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA206470.12457184649722153No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA201660.12166977558303721No Hit
AAGGTAGGTCCGGACGCTCGCGTTCGGACTGTTACAGCCCGGTTCTCTCG198220.11959428203942098No Hit
AAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAAGCCGGACGAC195560.11798939458999681No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG193410.11669221112523666No Hit
AAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTT190760.11509335708727648No Hit
TTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAGAAGCACT189650.11442364841477241No Hit
GAAATACCACTACTCTTATCGTTTTTTTACTTATTGGATGAAGTGGAAGC186420.1124748565119002No Hit
CCGGAGATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTC186080.1122697205221242No Hit
GAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATT184390.11125007398470807No Hit
AACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGAC183460.110688966718556No Hit
GGCTCGTAGCGATTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGG182200.10992875687409194No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT181710.10963311971235591No Hit
CCATTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAA181170.10930731549329986No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCAAATCG180700.10902374515449184No Hit
GCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTA180620.10897547786277983No Hit
GTAGCGATTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGA175260.10574156931807549No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA174920.10553643332829946No Hit
TTGAAGCCGGACGACGAATGAGAGTGCCTAGTGGGCCATTTTTGGTAAGC174490.10527699663534743No Hit
GTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACGG172410.10402204705083529No Hit
GGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATG171800.10365400895153126No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTG170570.10291189934145918No Hit
AAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGC170470.10285156522681918No Hit
GGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACG170400.10280933134657116No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT167810.10124667777739499No Hit
AATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGT167350.10096914085005096No Hit
TAATTGGAATGAGTACAACTTAAATCCTTTAACGAGGATCCATTGGAGGG166400.1003959667609709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)