FastQCFastQC Report
Wed 30 Apr 2025
2H2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2H2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20324284
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA302099214.863952894970373Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA9854664.848712013667984Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC4980542.450536510904886No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3976681.9566150522202896No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT3045991.4986948617722524No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT2716501.3365784497008604No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2022540.9951346871555229Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT940520.4627567691929516No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG810510.3987889561078757No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC779750.38365435161209127Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA759130.3735088527595855No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT726790.3575968531043947Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC687330.33818165500934744No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG679510.3343340409925388No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC638430.3141217668479736No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC631270.3105988875180056No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA624950.3074893068803802No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG493710.2429163064243739No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG491170.2416665699022903No Hit
CCTTTTAGGAGCGTCATATTTTACATTTTAGATCGTCGGACTGTAGAACT451640.22221693024954778No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT432780.21293739056195043No Hit
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA394960.19432910896147682Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT362310.17826458240792148No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA348980.17170592577824634No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC317650.15629086859837227No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG311930.15347650131242016No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT300860.1480298149740478No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT300580.14789204874326692Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG299960.14758699494653785No Hit
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT299570.14739510626795022Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATTCAGAAAAACTGAACAGTCATCAGTGCTGATCGTCGGACTGTAGAAC299360.14729178159486456No Hit
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC275650.13562593398124134Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG266250.13100092480502634No Hit
TATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGA257540.12671541098323563No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT243420.11976805677385732Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAACCGGTGTTCTCCCTGTGGTATGGTCGTAGGCGATCGTCGGACTGTA225320.11086245399837948No Hit
ATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGAC221340.10890420543227992No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG218370.1074428993414971Illumina Small RNA Adapter 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)