FastQCFastQC Report
Wed 30 Apr 2025
2F2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2F2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106837
Total Bases16 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA83157.782884206782295Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA31972.9924089968831025Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT14811.386223873751603Illumina RNA PCR Primer (100% over 50bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT9710.908861162331402Illumina RNA PCR Primer (100% over 44bp)
TCCATGAATTAATGAGCCGATCGATCGTCGGACTGTAGAACTCTGAACGT5730.5363310463603433Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT4200.39312223293428306Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC4040.3781461478701199Illumina DpnII expression Sequencing Primer (100% over 26bp)
CTACGCATTTCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG3800.3556820202738752Illumina RNA PCR Primer (100% over 33bp)
TGGCAACAGCTCGCAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA3770.35287400432434457Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAGAGCAAGCTCGTGAAGACACGATCGTCGGACTGTAGAACTCTGAAC3320.31075376508138564Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3130.29296966406769187No Hit
CAGCCCAGGCCGGCTCCACGATCGTCGGACTGTAGAACTCTGAACGTGTA2860.2676975205219166Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCTCAGCACTTACAATACGATCGTCGGACTGTAGAACTCTGAACGTGT2610.24429738760916161Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAACGT2340.2190252440633863Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGCAGATACGTTCTTCAGCAAGATGGATCGTCGGACTGTAGAACTCTGAA2340.2190252440633863Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCAGAGTTGTGCACATTCATGCTGCCAGAGGTCACGATCGTCGGACTGT2310.2162172281138557No Hit
TGGCAACAGCTCGCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT2110.19749712178365172Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTCTGAACGTGT1930.18064902608646816Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGAGTTTACAATTTATCGATCGTCGGACTGTAGAACTCTGAACGTGTAG1910.1787770154534478Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1820.170352967604856Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCATGAATTAATGAGCCGATCAGATCGTCGGACTGTAGAACTCTGAACG1730.16192891975626422Illumina Small RNA Adapter 1 (100% over 26bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC1620.15163286127465203Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCATCACAGACCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC1580.14788884000861124Illumina RNA PCR Primer (100% over 32bp)
CAGCCCAGGCCGGCTCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1580.14788884000861124Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1560.14601682937559085Illumina RNA PCR Primer (100% over 31bp)
ACCCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA1490.13946479216001947Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1400.13104074431142768Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCCGCAAGCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1370.12823272836189709Illumina RNA PCR Primer (100% over 31bp)
GTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTG1360.1272967230453869Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA1320.1235527017793461No Hit
CATGAATTAATGAGCCGATCGATCGTCGGACTGTAGAACTCTGAACGTGT1300.12168069114632571Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATGAGGTCAAGTTAGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1300.12168069114632571Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1270.11887267519679512Illumina RNA PCR Primer (100% over 37bp)
CCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACG1250.11700066456377471Illumina Small RNA Adapter 1 (100% over 26bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA1240.11606465924726451No Hit
TCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTCTGAACGTGTA1230.11512865393075433Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCATCACAGACCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1190.11138463266471353Illumina RNA PCR Primer (100% over 31bp)
TATTTGCTATACAGTCGGTCGGATCGTCGGACTGTAGAACTCTGAACGTG1140.10670460608216253Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCCAAAAGATCTATCACGATCGTCGGACTGTAGAACTCTGAACGTGTA1130.10576860076565237Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGAGTTTACAATTCATCGATCGTCGGACTGTAGAACTCTGAACGTGTAG1130.10576860076565237Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1110.10389659013263196Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCATGAATTAATGAGCCGATCATGATCGTCGGACTGTAGAACTCTGAAC1100.10296058481612175Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1080.10108857418310135Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATTCGATCAGTTACTATGGATCGTCGGACTGTAGAACTCTGAACGTGTA1080.10108857418310135Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGT1070.10015256886659117Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGATCG1070.10015256886659117No Hit
CCTCCCGTCCGAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1070.10015256886659117Illumina RNA PCR Primer (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)