FastQCFastQC Report
Wed 30 Apr 2025
1F7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607654
Total Bases541.1 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT2534837.026255843825378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC1741874.828262355536313Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA1470274.075418540691541Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGTATG734442.035782810657563TruSeq Adapter, Index 16 (97% over 43bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTAGATCGGAAGAGCAC725162.010059723022219No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG556041.5412786259436186No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGTGTG466511.2931118117203035TruSeq Adapter, Index 16 (97% over 43bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCCAGATCGGAAGAGCACACGTCT434761.2051044806403275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG420541.165688283854272No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCCAGATCGGAAGAGCACACGTCTGAA216740.6007782342763469Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA166410.4612692902368131No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGTGTG164370.45561464597214696TruSeq Adapter, Index 16 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGGGGG151590.42018996278467946TruSeq Adapter, Index 16 (97% over 43bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC133910.3711830458242393No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC119390.33093528370514463No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGTATG118190.3276090223729881TruSeq Adapter, Index 16 (97% over 43bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG116890.32400557259648516No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA114510.31740848762104124No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGGGGG112370.3114766549120287TruSeq Adapter, Index 16 (97% over 43bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC112040.31056193304568563No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC97440.27009242017111396No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCAGATCGGAAGAGCACACGTCTG96370.2671265038166077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGGGTG94190.26108379572985657TruSeq Adapter, Index 16 (97% over 43bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCAAGATCGGAAGAGCACACGTCT91490.2535997077325043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC85110.23591508498320515No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCG77800.2156526097014847No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTCAGATCGGAAGAGCAC72710.20154371788425385No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGTGGG69990.1940041921980323TruSeq Adapter, Index 16 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGGATG67340.1866586984228532TruSeq Adapter, Index 16 (97% over 43bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGTAGATCGGAAGAGCACACGTCTG65520.18161386873574906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGTATGCCGTC65350.1811426483803602TruSeq Adapter, Index 16 (97% over 39bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC61940.17169052242814858No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT61090.16933442065120435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGGGTG60100.16659025505217517TruSeq Adapter, Index 16 (97% over 43bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG60070.16650709851887124No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT57840.16032579620994697No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAAGATCGGAAG56730.15724900447770213No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGTAGG55230.15309117781250642TruSeq Adapter, Index 16 (97% over 43bp)
TACAAGTCCATAATAAGTTGGCTCCAAGAACTTAGATCGGAAGAGCACAC54410.1508182325688661No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTACAAATCAGTTAGATC52270.14488639985985352No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT51920.1439162403046412No Hit
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGCCAGATCGGAAGAGCA49720.1378180945290208No Hit
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT49620.13754090608467442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAT46700.12944700350976007TruSeq Adapter, Index 16 (97% over 41bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTCTTAAGATCGGAAGA45590.12637021177751526No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAGATCGGAAGA44110.12226782280118881No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC43620.12090959942389154No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT42380.11747246271399642No Hit
TGAAAATCTTTTCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC41150.11406304484853592Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC40030.11095853427185644No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC39730.11012696893881731No Hit
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGCTAGATCGGAAGAGCA38660.10716105258431101No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTTAGATCGGAAGAGCACACGTC38590.10696702067326856No Hit
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG38260.1060522988069255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT37180.10305866360798457No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG36870.10219937943051079No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC36290.10059168645330178No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGTGGG36240.1004530922311286TruSeq Adapter, Index 16 (97% over 43bp)
CTTGATGTTTGAGCTGAGCAGAAGCTACTTGACAGATCGGAAGAGCACAC36080.1000095907201744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)