FastQCFastQC Report
Wed 30 Apr 2025
1E7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271172
Total Bases40.6 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGG6931325.56052984821442TruSeq Adapter, Index 15 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGGGGG163486.028646025400852TruSeq Adapter, Index 15 (97% over 43bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC124844.603720148097886Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGTG104943.8698685705013793TruSeq Adapter, Index 15 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGGGGG42971.5846031301166785TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGGGTG40311.4865104066791557TruSeq Adapter, Index 15 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGGGGT20700.7633531485551606TruSeq Adapter, Index 15 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGCGGT18580.6851739855147286TruSeq Adapter, Index 15 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGCGTT18000.6637853465697049TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGTGGG17780.6556724145560751TruSeq Adapter, Index 15 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGTGTG15370.5667989320431313TruSeq Adapter, Index 15 (97% over 43bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAAT14280.5266030416119658TruSeq Adapter, Index 15 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGCGGG10510.387576888469311TruSeq Adapter, Index 15 (97% over 39bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG9720.35844408714764064No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG9700.3577065478736743No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGGGTT8810.3248860501821722TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGTGGG8010.2953844792235186TruSeq Adapter, Index 15 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGT7820.28837785612083844TruSeq Adapter, Index 15 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGTGTG7490.27620845810039385TruSeq Adapter, Index 15 (97% over 43bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG6880.2537135102444205No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC6870.2533447406074374No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGTGGCGTT6360.2345374891212957TruSeq Adapter, Index 15 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGTCGTT5740.2116737716283392TruSeq Adapter, Index 15 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGGGTG5650.2083548448954907TruSeq Adapter, Index 15 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGTGTCGTT5140.18954759340934904TruSeq Adapter, Index 15 (97% over 39bp)
TGAAAATCTTTGCTCTGAAGTGGTAAGATCGGAAGAGCACACGTCTGAAC5010.18475358812856785Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGAGG4900.18069712212175298TruSeq Adapter, Index 15 (97% over 43bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGG4800.17700942575192127TruSeq Adapter, Index 15 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGGAGG4740.17479680793002228TruSeq Adapter, Index 15 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGCGTC4570.1685277241013084TruSeq Adapter, Index 15 (97% over 39bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC4530.1670526455533757No Hit
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT4440.1637337188205272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGTT4430.16336494918354402TruSeq Adapter, Index 15 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGGGGGGCGTT4430.16336494918354402TruSeq Adapter, Index 15 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGGGGGGCGGT4380.16152110099862818TruSeq Adapter, Index 15 (97% over 39bp)
TGAAAATCTTTGCTCTGAAGTGGGAAAGATCGGAAGAGCACACGTCTGAA4250.15672709571784696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGG4220.15562078680689748TruSeq Adapter, Index 15 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGTGTT3930.14492646733438555TruSeq Adapter, Index 15 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGCGTG3760.1386573835056717TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTAGGGGT3740.13791984423170534TruSeq Adapter, Index 15 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGCGGC3720.137182304957739TruSeq Adapter, Index 15 (97% over 39bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT3650.1346009174988568Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC3610.13312583895092414No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGTGGT3470.12796306403315977TruSeq Adapter, Index 15 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGTGGCGGT3390.1250129069372944TruSeq Adapter, Index 15 (97% over 39bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA3160.11653120528668152No Hit
GATCGGCTCATTAATTCATGGAAGATCGGAAGAGCACACGTCTGAACTCC3060.11284350891684983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGTCGGT3000.11063089109495079TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGG2970.10952458218400131TruSeq Adapter, Index 15 (96% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTGGGGGGGTGGG2850.10509934654020327TruSeq Adapter, Index 15 (97% over 39bp)
GTGTGCTCAGGATCTGTGACGTTTACTTTAGATCGGAAGAGCACACGTCT2820.10399303762925376Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)