FastQCFastQC Report
Wed 30 Apr 2025
1B7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269760
Total Bases40.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGTG3265412.104833926453143TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGGG2740710.159771648873072TruSeq Adapter, Index 14 (97% over 43bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC230418.541295966785292Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGG139375.166444246737841TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGGG128984.781287069988138TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGTG123654.58370403321471TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTATG111604.137010676156583TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGTGTG47741.7697212336892052TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGAGG35731.3245106761565837TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTAGG31521.1684460260972716TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGATG29191.082072953736655TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGTG28061.0401838671411625TruSeq Adapter, Index 14 (97% over 43bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT26800.9934756820877817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGTGGG24340.9022835112692764TruSeq Adapter, Index 14 (97% over 43bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA13480.4997034400948992Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAT12030.4459519572953737TruSeq Adapter, Index 14 (97% over 41bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG11570.4288997627520759No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGTT11420.4233392645314354TruSeq Adapter, Index 14 (97% over 43bp)
CAGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCT9490.35179418742586TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGTATG9120.33807829181494664TruSeq Adapter, Index 14 (97% over 43bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG7330.27172301304863583No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT6900.2557829181494662Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTG6490.24058422301304863TruSeq Adapter, Index 14 (97% over 43bp)
TTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT6090.22575622775800713Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGTGCCGTC5660.20981613285883746TruSeq Adapter, Index 14 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGTGTCGTC5580.2068505338078292TruSeq Adapter, Index 14 (97% over 39bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGG5570.20647983392645314TruSeq Adapter, Index 14 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGTGTCGTT5100.18905693950177935TruSeq Adapter, Index 14 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGAGG4510.16718564650059312TruSeq Adapter, Index 14 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGGGGGGG4390.16273724792408067TruSeq Adapter, Index 14 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTATGCCGTC4080.15124555160142347TruSeq Adapter, Index 14 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTATT4030.1493920521945433TruSeq Adapter, Index 14 (97% over 43bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC4010.14865065243179124No Hit
TAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAG4000.14827995255041518TruSeq Adapter, Index 14 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGTGGCGTC3610.13382265717674968TruSeq Adapter, Index 14 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGTAGG3510.13011565836298933TruSeq Adapter, Index 14 (97% over 43bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA3370.1249258600237248No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGTGGCGTT3320.1230723606168446TruSeq Adapter, Index 14 (97% over 39bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTAGATCGGAAGAGCAC3040.11269276393831554No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGATG3030.1123220640569395TruSeq Adapter, Index 14 (97% over 43bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG2880.10676156583629894Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTGGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC2750.10194246737841044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGTGCCGTT2710.1004596678529063TruSeq Adapter, Index 14 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)