FastQCFastQC Report
Thu 20 Feb 2025
POR-83-TP4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-83-TP4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23548689
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA1665170.7071179206621651No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG1171270.4973822534239592No Hit
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG1069770.4542800662915885No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT1061160.45062381179691147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1040650.44191419743154275No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC865790.3676595329786724No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT859470.3649757317700361No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG736420.31272229209872365No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG731300.31054807339805623No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT663780.2818755642830053No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC658260.2795314847463483No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAA648090.27521277299131175No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT634390.26939503935866665No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA580160.2463661565193714No Hit
AGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAA566760.24067581851371853No Hit
GAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAAC554460.23545259780703717No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC532050.22593614447071766No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA491090.20854239486537868No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA484410.20570571890435174No Hit
TGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGG471080.20004510654499705No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAG468790.19907265325895634No Hit
GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC467450.1985036194583911No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT441260.1873819812219695No Hit
TTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGC440190.18692760348569723No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT434550.18453256569824333No Hit
AAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAG418800.1778442952811513No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC415400.1764004781752394No Hit
CGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTG413640.175653090496885No Hit
ATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAG411540.17476132110793938No Hit
CAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGA408970.17366996523670597No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAA406350.17255737676097382No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA404990.17197984991860904No Hit
ACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGAT404600.17181423560351916No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAAC397970.1689987922469909No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA396200.16824715804773677No Hit
GTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCT387970.16475227134724993No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA387440.16452720573956367No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG381540.16202175840871652No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA380070.1613975198364546No Hit
GTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGA357660.1518810665001351No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA354410.15050094720771928No Hit
CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATA342570.145473066462426No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA342140.14529046606373713No Hit
CGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTA338060.1435578855366428No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG333350.14155777419286483No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACC328690.13957889545358557No Hit
GACTAATCGAACTGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA321300.13644071650867698No Hit
TCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATG320290.13601181789780314No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGG311570.13230885167322903No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGA311350.1322154282134347No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG308530.13101790931970778No Hit
CTCTTATCGTTTTTTTACTTATTGGATGAAGTGGAAGCGGACCGCAAGGT308180.13086928108821685No Hit
ATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTA304370.12925135662541554No Hit
TAACAATACAGGGCTTTTACAAGTCTTGTAATTGGAATGAGTACAACTTA304040.12911122143572407No Hit
GTGAAATTCTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCA303240.1287714997637448No Hit
ATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAG302520.12846575025896345No Hit
CAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAAC302330.1283850663618684No Hit
CTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAG299320.12710686357104636No Hit
GGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTC297140.1261811220149028No Hit
CGGGAATGCAGCCCAAAAATGGTGGTAAACTCCATCTAAAGCTAAATACT292690.1242914202145181No Hit
CTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCAT292100.12404087548143337No Hit
TTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAAT290500.12336143213747483No Hit
GTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAA289680.12301321742369607No Hit
CAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCG287960.12228281582894063No Hit
CGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCG287850.12223610409904347No Hit
CGAACAACGAACTTAGAACTGGCAGTGACTAGGGGAATCCGACTGTTTAA285090.12106406433071498No Hit
GTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCT283590.12042708619575382No Hit
GTTTATTTGATTGTACCATTACTACTTGGATAACCGTAGTAATTCTAGAG277700.1179258853858064No Hit
CCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAT269320.1143673008718235No Hit
GGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA267240.11348402452467736No Hit
GTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCT267180.11345854539927891No Hit
TCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAG265640.11280458118071882No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGA262060.11128432669861153No Hit
TGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATT261530.11105926109092527No Hit
CAAATTTCTGCCCTATCAACTGTCGATGGTAAGGTAGTGGCTTACCATGG260850.11077049766974288No Hit
CTCACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTT260000.11040954339326492No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC258740.10987448175989756No Hit
GCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGG255540.10851559507198046No Hit
CTCAGCAATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG254820.10820984556719909No Hit
CCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAA250590.10641356722660866No Hit
GTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTAT250020.10617151553532343No Hit
AAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGG249950.10614178988902524No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG249190.10581905430064494No Hit
TTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAAC247520.10510988531038819No Hit
CCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTG241160.10240909801815293No Hit
GTTGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAG236720.10052364273866796No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)