FastQCFastQC Report
Thu 20 Feb 2025
POR-83-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-83-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19283722
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1482970.7690268507293353No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1133700.5879051772266786No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA810160.42012636357234356No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC726580.37678410837907744No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC700340.3631767767653983No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA680160.35271199201067094No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG622430.32277482531639895No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC548310.2843382620844669No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC483420.25068811923341355No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA475340.24649805675481112No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA456450.2367022299948112No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC443870.230178593115997No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA438310.22729533230151314No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA415760.21560153169600765No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA413020.2141806441723232No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG412420.2138695009189616No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC381460.19781450904550482No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC380720.1974307656996922No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC375530.19473937655811466No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC373860.19387336116959164No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG368320.19100047179688653No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT362130.18779051056637303No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC346320.17959188584029578No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC340110.17637155316800357No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA321780.16686612677780774No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC317210.16449625233137047No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT315750.163739137081524No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA305430.15838747312370505No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG299590.15535901212431916No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT284550.14755968790672258No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT281360.1459054429430169No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC280540.14548021383008944No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC265360.1376082895200418No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG261040.13536805809583854No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC256150.13283224058094178No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG255090.13228255416666967No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT253860.1316447104972785No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT253060.13122985282612973No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG249360.12931113609706674No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC248000.12860587805611387No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG243280.1261582177963362No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT236950.12287565647337169No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA235980.12237264154710382No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA233620.121148811417215No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT231720.1201635244482367No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA229390.11895525148101595No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT229330.11892413715567982No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT227790.11812553613871844No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT225830.11710913484440401No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT225750.11706764907728913No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT224530.11643499112878727No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC220920.11456294588772852No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG218730.11342727301295881No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA218650.11338578724584393No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG216010.11201675693105304No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT215880.11194934255949138No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC215150.11157078493456814No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC210350.1090816389076756No Hit
CACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGATACCAACAGTCCCT209780.10878605281698212No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC204970.10629172106920023No Hit
GAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG204380.10598576353672802No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC204000.10578870614293236No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCA200810.1041344611792267No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCCA194910.10107488585450464No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA194590.10090894278604515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)