FastQCFastQC Report
Thu 20 Feb 2025
POR-83-TP1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-83-TP1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18220049
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1510730.8291580335486474No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1123240.6164857185620083No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC861700.47294055026965076No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA708860.3890549361310719No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG668270.36677727924880993No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC594380.3262230524187943No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA549080.3013603311385167No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC518710.2846918798077876No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA507410.2784899206363276No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC506390.27793009777306305No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA503770.27649212139879537No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG477280.2619531923322489No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA436650.2396535816122119No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA408210.22404440295413036No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT392940.21566352538349376No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC366240.20100933866862816No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC363520.19951647769992278No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG362040.19870418570224485No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA359070.19707411324744517No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA357010.19594349060202856No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG349100.1916021191820066No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG341220.18727721314031592No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC328760.18043859267337864No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA325540.17867130873248477No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA314250.17247483802046856No Hit
TTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCG303770.16672293252339773No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC293780.1612399615390716No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG291400.15993370819145436No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC290040.15918727770710167No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC288610.15840242800664256No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC285960.15694798625404355No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT280070.1537152836416631No Hit
GATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGT279150.15321034537283626No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT278760.15299629545452922No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC278290.15273833786067206No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC272720.14968126595049225No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT269460.14789202817182323No Hit
CAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCT264280.14504900617995045No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC264100.1449502139099626No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT261700.1436329836434578No Hit
CTTTATTTAAGTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTT254540.1397032466817186No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC253890.13934649681787353No Hit
GAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG253700.13924221608844192No Hit
CTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGC252280.1384628548474266No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG251470.13801828963248122No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA249020.1366736170687576No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG239120.13124004221942542No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA238550.13092720003113054No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG230020.12624554412559483No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCA224290.12310065686431469No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC222250.12198101113778563No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT218830.12010395800801632No Hit
ATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTT218230.11977465044139014No Hit
CTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAG214260.1175957320422135No Hit
CTGATCCCTAGTTGGCATCGTTTATGGTTAGAACTAGGACGGTATCTGAT207980.11414897951152601No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT206440.11330375675718544No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC206330.11324338370330399No Hit
CTTCAAACGGTTTCACGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCA204340.11215118027399379No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCCA202390.11108093068245865No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG199590.10954416203820308No Hit
CACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGATACCAACAGTCCCT199550.10952220820042799No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT198680.10904471222882002No Hit
CTTGTCACTACCTCCCTGAGTCAGGATTGGGTAATTTGCGCGCCTGCTGC195850.10749147820623316No Hit
ATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGG193690.10630597096637885No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT192190.10548270204981337No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA188150.10326536443453034No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC187880.10311717602954854No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG187730.10303484913789199No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC187270.10278238000347859No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG186510.10236525708575206No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT185770.10195911108691309No Hit
CTTTTTAACTGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCG185180.10163529197973067No Hit
CTTTACCAGGCATTAAACCCTATCTCCGGACAATCCGATTTCAGGGTGAA182940.10040587706432623No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC182700.10027415403767576No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)