FastQCFastQC Report
Thu 20 Feb 2025
POR-74-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-74-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18048084
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1586890.8792567676435903No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1075130.5957031228356429No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC967350.5359848724108331No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA854790.473618141404927No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA634680.35166059732434757No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG625490.34656864407324345No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC619010.34297823525200793No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA568630.31506391481777235No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG547170.3031734559746065No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC534500.2961533202083944No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA506310.28053393368514906No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT502680.2785226398547347No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA501700.2779796459280664No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC497570.27569131437996414No Hit
GCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAAC475040.2632079948209461No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA470770.2608420927118912No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA466100.25825456042868594No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC420960.23324359527581986No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC412690.22866139142526154No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC409560.2269271353125351No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA400270.22177977451789344No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC392880.21768515705046587No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC377890.20937956627418178No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA375470.20803870372057223No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC364500.2019604961944991No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT362420.2008080192889173No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC359590.19923998580680366No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA351460.19473535251719795No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG345380.19136657387011274No Hit
CTTTACCAGGCATTAAACCCTATCTCCGGACAATCCGATTTCAGGGTGAA341890.18943285060065101No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC336450.18641868023220637No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC330730.18324936874185646No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC329510.18257339671069794No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG318370.1764009963606109No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG314040.17400184972543345No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA313180.17352534485101023No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC303290.16804553879514303No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG299540.16596775591248356No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG298020.16512556125071226No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT297070.1645991895871052No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA287700.15940750275763343No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT284550.1576621651361995No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT281550.15599993883007193No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT272970.15124597159454709No Hit
CTTCAAACGGTTTCACGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCA260910.14456382184391428No Hit
CTGATCCCTAGTTGGCATCGTTTATGGTTAGAACTAGGACGGTATCTGAT260600.1443920584589478No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC259910.14400974640853842No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG254090.14078502737465096No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC253250.14031960400893523No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCCA252930.14014229986961496No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT248020.13742178948191952No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT247140.1369342030987888No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG246170.1363967499264742No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT239780.13285620789442248No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC236400.13098343292285208No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG235540.13050692804842884No Hit
CTCTGATCCCTAGTTGGCATCGTTTATGGTTAGAACTAGGACGGTATCTG235100.13026313485686347No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT234960.13018556429591085No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC234820.13010799373495824No Hit
CTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGC234570.1299694748761143No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG233810.12954837754522863No Hit
TTCAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCC231970.12852887874413704No Hit
CCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTT231630.12834049309610926No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCA230560.1277476323802571No Hit
CTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACA227650.12613527286331336No Hit
GAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG227350.1259690502327006No Hit
GGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGG227320.12595242796963935No Hit
TCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCA226760.12564214572582885No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT225780.1250991517991605No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC225190.12477224729228877No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT223570.12387464508697987No Hit
TTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCG219920.121852269747858No Hit
GTTTATGGTTAGAACTAGGACGGTATCTGATCGTCTTCGATCCTCTAACT219870.12182456597608922No Hit
CTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGC219820.12179686220432041No Hit
CACGGTACTTGTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGG219510.1216250988193539No Hit
TAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCT219200.12145333543438737No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC218660.12115413469928442No Hit
CTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTG216080.11972462007601471No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA214850.11904310729050241No Hit
CCCAGTCCCAACCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGG214720.11897107748390355No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC214170.11866633599444683No Hit
GTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCAC213320.11819537187437736No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC212450.11771332624560037No Hit
CCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACG209310.11597352937852018No Hit
CTTGTCACTACCTCCCTGAGTCAGGATTGGGTAATTTGCGCGCCTGCTGC209300.11596798862416643No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC208110.11530863885606914No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC208020.11525877206688533No Hit
CCTCACGGTACTTGTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGA205110.1136464125499416No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT204300.11319761144728716No Hit
CTTTTTAACTGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCG203510.11275989185334022No Hit
CAACAGACACGCTCTTACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTC203190.11258258771401994No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT202910.11242744659211472No Hit
ATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGG202470.11218365340054932No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG201980.11191215643721517No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG196100.10865419287720514No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA193310.10710832241250651No Hit
CACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGATACCAACAGTCCCT193230.10706399637767643No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT192220.1065043801879468No Hit
GTCAACGATGCGTCCGGCTACGAGAGTCGGAATCTCGCCTCAGCGGCCGA191560.10613869040059876No Hit
TCAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCA190380.10548488138685526No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC189560.10503053952984705No Hit
CTTTATTTAAGTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTT188430.10440443428787233No Hit
GTTCCAAGCAACTTAGGCACGACCGATCCGCCTAGAAAGTGCTTCTCGCA187970.10414955958759943No Hit
CTGCTGTTCACATGGAACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATT185840.10296937891024886No Hit
CACAGATCCTGAGCACACTCAGTTAAGAGCACACACAGTCTTTGCGAAGA184530.10224354008990649No Hit
CGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCA181800.10073091415133043No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)