FastQCFastQC Report
Thu 20 Feb 2025
POR-73-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-73-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19415470
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1881080.968856278009237No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1512400.7789664633408308No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA1126280.5800941208222103No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC1061990.5469813504385935No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC961850.4954039227482002No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG823340.4240639036809307No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC742100.38222098151628575No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA680060.3502670808381152No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC676340.34835108292511074No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA630630.32480800104246765No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG625830.32233574567084905No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA555700.2862150645850963No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA551170.28388187357813127No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC530420.273194519627905No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC521550.26862599772243473No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA495400.2551573564791375No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA453780.23372084219439446No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC426330.21958263178795054No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG424000.21838255782631066No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT415850.21418487422658322No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC412740.21258305876705533No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC411520.21195469386010227No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG406260.2092455140153702No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT397150.20455337934131906No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC395960.2039404660304386No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC392800.2023128979107897No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT385390.19849635368085344No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA383410.19747654834006081No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC362450.18668103321732618No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC356600.18366797198316598No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA353440.18204040386351708No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT349590.18005744903419799No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT347840.17915610592996203No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT343270.17680231279490016No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC339460.17483996009367786No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC329930.16993150307461008No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG329350.16963277221720618No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG328000.16893745039393843No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT326720.16827818229484015No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC326640.1682369780386465No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA320560.16510545456792958No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC315710.16260744653618994No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT315480.16248898429963324No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT314580.16202543641745476No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG313180.16130436193406597No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA308140.15870849379386642No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC294830.15185313566964898No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG293310.15107025480196976No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT293070.15094664203338884No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA292900.15085908298897735No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC289690.1492057622092074No Hit
GTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCC287440.14804689250376118No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT284990.14678501215783082No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC283080.1458012605412076No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC281800.14514199244210932No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA281050.1447557025402939No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT280360.1444003158306237No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG279150.1437771014556949No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC272500.14035199765959824No Hit
CTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGC270230.1391828268901036No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA269730.13892530028889336No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG268890.1384926555988601No Hit
GAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG265790.13689599067135638No Hit
GTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTA264350.13615431405987083No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT263190.13555685234506298No Hit
CTTCAAACGGTTTCACGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCA261520.13469671349702067No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC261120.13449069221605245No Hit
GGACGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTG259510.13366145656015538No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT257870.1328167693081857No Hit
CCCAGTCCCAACCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGG256690.13220900652932943No Hit
CAACAGACACGCTCTTACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTC255430.13156003949427955No Hit
CTGCTGTTCACATGGAACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATT254640.13115314746436732No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA249980.1287529995410876No Hit
GCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAAC249330.12841821495951425No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT247660.12755807611147194No Hit
CCGCATTCGACAACCGGCCGGAAGGACGGGACCCTAGCCACAAGAGGCAT245930.12666703407128438No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC244910.12614167980481544No Hit
GCGGTTATGAGTACGACCGGACACGAAAATCATTGTCTCCCGCGGATTTT244230.12579144362716949No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG241720.1244986600890939No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA241390.12432869253229512No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCA241230.12424628401990784No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG235500.12129502917003812No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT234630.12084693288393225No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTACCTACATTGTTCTA231940.11946143976942099No Hit
CTTTATTTAAGTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTT231900.11944083764132415No Hit
GTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTG231660.11931722487274322No Hit
CGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCC230100.11851374187696719No Hit
CACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGATACCAACAGTCCCT224010.11537706787422607No Hit
CTAGGCACTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCT223360.11504228329265272No Hit
CCTATACCCAAATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCC222560.11463024073071627No Hit
ATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGCACTTTGGGACGCAT222010.11434696146938499No Hit
CGGCGCATTGCTGCTCCCACTTATTCTACACCTCTCATGTCTTTTCACAA220180.1134044141089554No Hit
GCCATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGC219180.11288936090653484No Hit
GTTAAAAAGAAAAGAGAACTCTTTCCGGGGCCCCCGCCGGCGTCTCCGCG217240.11189015769383899No Hit
TGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTTTT216250.11138025502344265No Hit
CTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAG214150.11029864329835949No Hit
CTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTG211430.10889769858777563No Hit
CCATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCT210560.10844960230166976No Hit
TAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCT208120.10719287248776362No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCA207490.10686838897023868No Hit
CGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGT207440.10684263631011764No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG207350.1067962815218998No Hit
GTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGT207060.10664691609319785No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC205900.10604945437839003No Hit
GGGGTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCAT204010.10507600382581518No Hit
CTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAAT203230.10467426232792715No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC202870.10448884317505577No Hit
GTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCAC202370.10423131657384549No Hit
TGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCA201890.10398409103668364No Hit
CTTTTCACAAGGTCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC200400.10321666176507703No Hit
GCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGC199680.10284582345933423No Hit
CCACCGTCCTGCTGTCTATAGCAACCAACACCTTTTGTGGGGTCTGATGA199150.10257284526205133No Hit
CATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTT197780.1018672223747352No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC194950.10040962181188506No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)