FastQCFastQC Report
Thu 20 Feb 2025
POR-73-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-73-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21791714
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA2001160.9183123456924958No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1586280.7279280555903037No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA1095810.5028562691305511No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC1030690.4729733512471758No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC940940.43178797225404114No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA838630.38483893465195074No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG785430.36042598576688367No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC698650.3206035101231597No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA628400.2883664864544386No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC615770.28257070554431835No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC606210.2781837169852725No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA602980.2767015022315362No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA554770.2545784145294858No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG532330.24428092255616057No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA529490.24297767490891264No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA524100.24050425771924136No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG493770.22658612351465335No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC473660.21735784528009133No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC472350.2167566993582974No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC471280.21626568704049623No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC468820.21513681759957018No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC443940.2037196339856516No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT442720.20315978816535496No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC431970.19822672048651152No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA416860.19129289233513252No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT416190.190985436023986No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG411300.18874146384263302No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC407020.1867774145714284No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT392770.18023823183435683No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA390720.17929750730025185No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC373550.17141836571460142No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT370450.16999580666302797No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT363160.16665049844174717No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT355310.16304821181114987No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG349950.1605885613219777No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA344800.1582252777362992No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC342560.15719736409903323No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC341930.15690826338855218No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG339560.15582069404912344No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA335440.15393006718058064No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG334130.15332892125878672No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT330450.15164020599756403No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC327890.15046544755497432No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG320130.14690446102587434No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT319780.14674384952005154No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA312870.14357291950509263No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC310590.14252665026716116No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT305690.14027808918564186No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC305080.13999816627549352No Hit
CACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGATACCAACAGTCCCT300170.13774501629380784No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT298960.13718975937367756No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG294930.13534043260663206No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT292490.13422074096603875No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA286510.13147657866655188No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT286040.13126090035873267No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC284600.130600098734776No Hit
GAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG284260.1304440761291195No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG284000.130324764724794No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC283630.13015497541863846No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT282710.1297327966033328No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG270980.12435001670818552No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT269410.12362955938206605No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC265520.12184447721734967No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCA264660.1214498318030422No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC260990.11976570544198588No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC260350.11947201583133846No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA254420.11675079803268344No Hit
GGACGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTG250210.11481887106264334No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCCA249690.11458024825399232No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG248680.11411676933718935No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT244810.11234086497280571No Hit
CTTTATTTAAGTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTT243740.11184985265500456No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA242770.11140472933886705No Hit
CTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGC242690.11136801813753612No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC242620.11133589583637157No Hit
GTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTA231830.10638447255686266No Hit
GATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGT229990.10554011492625133No Hit
GTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCC229870.10548504812425492No Hit
CTGCTGTTCACATGGAACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATT229510.10531984771826575No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT228930.10505369150861654No Hit
TTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCG228620.10491143560345918No Hit
CAACAGACACGCTCTTACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTC228390.10480589089963278No Hit
CGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCC224240.1029014973305909No Hit
CTTCAAACGGTTTCACGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCA224030.1028051304270972No Hit
CCCAGTCCCAACCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGG222460.10208467310097774No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC219570.10075848095289797No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG218970.10048314694291602No Hit
CCGCATTCGACAACCGGCCGGAAGGACGGGACCCTAGCCACAAGAGGCAT218290.10017110173160312No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC218100.10008391262844216No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)