FastQCFastQC Report
Thu 20 Feb 2025
POR-69-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-69-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21158221
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA2077810.9820343591268851No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1625610.7683112866625224No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC1069730.5055859847574141No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG949840.4489224306712743No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA904700.4275879337870608No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC890240.42075371081529017No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA862520.40765242030509086No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC692800.32743773684942606No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA682120.32239005349268257No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA653290.30876414420664194No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC611890.2891972817563443No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA591140.27939021905480615No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA574200.2713838748541288No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG557610.26354295098817615No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC535300.2529985862232935No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG509240.24068186072921724No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT483930.22871960738097974No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC482140.2278736005262446No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA481430.22753803356151728No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC479020.22639899639955555No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC454120.2146305211577098No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC433530.20489907918061734No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA432000.20417595600310628No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG430460.20344810653031747No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC426120.20139689437973068No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC422960.19990338507193023No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG421060.19900538896913877No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG411690.1945768502937936No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA405920.19184977791847432No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC404000.1909423292251272No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA402250.1901152275515035No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC389030.18386706519418622No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG383520.18126287649609105No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT367680.17377642477597713No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC366300.17312419602763388No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC360310.17029314515620192No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG350360.1655904813547415No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT350310.16556684987835224No Hit
GAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG347680.16432383422027777No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT342410.161833076608851No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA339060.16024976769077134No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT332550.15717294946489122No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC325280.1537369327978945No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG324130.1531934088409418No Hit
GATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGT324020.15314141959288544No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT323990.1531272407070519No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC318630.15059394643812446No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT306750.14497910764803904No Hit
TTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCG305530.14440249962414137No Hit
CTTTATTTAAGTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTT302710.14306968435578776No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT300960.14224258268216405No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT300450.14200154162299372No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT299220.1414202073038182No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA295820.13981326690934934No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA291620.13782822289265245No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT289960.13704365787652942No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG287990.13611257770679303No Hit
CACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGATACCAACAGTCCCT285590.1349782668401091No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC283240.13386758744981442No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCA281620.13310192761480277No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT279920.13229845741756832No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC279350.1320290585867309No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC273650.12933507027835656No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT272840.12895224036085076No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCCA270480.12783683467527823No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG270190.1276997721122206No Hit
CTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGC267360.1263622305485891No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT265740.1255965707135775No Hit
CAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCT264350.12493961566995637No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG263240.12441499689411506No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC257630.12176354524324139No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC249900.11811011899346359No Hit
CTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAG248470.11743425876873108No Hit
CTGCTGTTCACATGGAACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATT245870.11620542199649016No Hit
GTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAA244880.11573751876398303No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC244390.1155059302953684No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC243340.1150096692911942No Hit
CAACAGACACGCTCTTACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTC239070.11299154120755237No Hit
CCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTT232300.10979183930444814No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA232220.10975402894222534No Hit
CGGCGCATTGCTGCTCCCACTTATTCTACACCTCTCATGTCTTTTCACAA231770.1095413456547221No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT230530.10895528504026875No Hit
TGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAA229450.10844484515026098No Hit
CGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATG228390.10794385785080891No Hit
ATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGG226940.10725854503552071No Hit
CTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGC223700.10572722536549742No Hit
CGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCC223410.10559016280243975No Hit
CCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACG222400.10511280697937694No Hit
GCGGTTATGAGTACGACCGGACACGAAAATCATTGTCTCCCGCGGATTTT221930.10489067110131803No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC221360.10462127227048058No Hit
CCGCATTCGACAACCGGCCGGAAGGACGGGACCCTAGCCACAAGAGGCAT220330.10413446385686208No Hit
TTCAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCC219800.10388397020713604No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC219760.10386506502602463No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC219740.10385561243546895No Hit
CAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTG219470.10372800246296698No Hit
ATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTT219090.1035484032424087No Hit
TGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTTTT218110.10308522630517944No Hit
GCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAAC215740.10196509432432906No Hit
TAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCT215540.10187056841877208No Hit
CGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCA213270.10079769939070019No Hit
AACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAG212150.10026835431958103No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)