FastQCFastQC Report
Thu 20 Feb 2025
POR-260-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-260-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17191159
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1189710.6920475809687991No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC899010.5229490344426457No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA701810.4082389093137932No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC631270.36720619011202216No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC606760.3529488616794249No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA504740.293604404449985No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC500930.29138814898983834No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG451100.2624023197039827No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC434020.2524669802658448No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA408480.2376105066563575No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG391410.2276809841616845No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA382980.22277730082073No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC352650.20513451129153074No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC344780.20055657678461353No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA334250.1944313353160191No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA330940.19250592702912003No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC327010.1902198682473939No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG319200.18567683540126642No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC315880.18374561017090238No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA312610.1818434696578631No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC306750.17843474078740124No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT296560.1725072753966152No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC289750.1685459368969829No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA278900.16223455323751004No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT273430.15905268516218132No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT268640.15626636924246934No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT243940.141898518884038No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA234130.13619209734492013No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC234040.1361397448537356No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC233480.13581399601969826No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC232700.13536027442943202No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA228690.13302768009998628No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT228680.13302186315652134No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT223810.13018901168908972No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT220900.1284962811407887No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG218430.12705949610494557No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC216440.12590192435542014No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG212100.12337737089163098No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA211900.12326103202233195No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC206470.12010243172086305No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT204630.11903211412331188No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG201910.11744990550084494No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT200310.11651919454645263No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA198360.11538489057078699No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC193400.11249968661217083No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT192020.11169694841400746No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC191650.11148172150580422No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC188920.10989369593987235No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT187690.10917821189368325No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA186590.10853834811253855No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA186110.10825913482622085No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG184170.10713064779402016No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC180400.10493766010773327No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG180330.10489694150347861No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT178160.10363466477158405No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC176960.1029366315557898No Hit
GGACGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTG176900.1029017298950001No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT176310.10255853023056792No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG175940.10234330332236471No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG174280.10137769070718268No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT173860.1011333790816547No Hit
GTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCC173570.10096468772117109No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT172410.10028992227923667No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC172160.10014449869261287No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC171920.10000489204945404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)