FastQCFastQC Report
Thu 20 Feb 2025
POR-245-TP3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-245-TP3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18939312
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1606530.8482515098753323No Hit
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA1312970.6932511592818156No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG1108830.5854647729547937No Hit
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG879170.4642037683311833No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG674330.35604778040511714No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG603530.3186652186732021No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT572310.3021809873558237No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC516900.272924380780041No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT492940.260273446047037No Hit
AGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAA485670.25643486943981914No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA452360.2388471133481512No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT426340.22510849390938803No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT424920.22435873066561238No Hit
GAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAAC409540.21623805553232348No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC393110.207562978000468No Hit
GTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCT387490.20459560516242614No Hit
ATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAG383770.20263143666464759No Hit
TTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGC381130.201237510633966No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA377050.19908326131382173No Hit
GTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGA376590.19884038026302114No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA368020.19431540068614953No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG363180.19175986962989996No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAA358430.18925185877924183No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT350280.1849486401617968No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC349760.18467407897393529No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA349670.18462655876834386No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA336830.1778470094373016No Hit
AAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAG331450.17500635714750357No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC327440.17288906798726375No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA313330.16543895575509818No Hit
CGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTG307040.16211782138654246No Hit
GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC305630.16137333816561023No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA299290.15802580368283706No Hit
GTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCT290770.1535272242201829No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA289220.1527088206794418No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA288540.1523497791260844No Hit
CAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGA287390.15174257649908296No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGA286330.15118289407767294No Hit
TGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGG282740.14928736587685973No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAA279050.14733903744761162No Hit
ACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGAT278690.14714895662524596No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT278650.147127836533872No Hit
GGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA276250.14586063105143418No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG268820.14193757407872049No Hit
GACTAATCGAACTGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA266060.14048028777391702No Hit
GTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCT264810.13982028491848067No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGG264620.13971996448445434No Hit
CTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCAT257380.13589722794576697No Hit
CGAACAACGAACTTAGAACTGGCAGTGACTAGGGGAATCCGACTGTTTAA254270.13425514084144133No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAG253490.13384329905964906No Hit
CAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCG246020.12989912199556142No Hit
TGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAG242220.12789271331503488No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC241310.12741223123627723No Hit
CGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCG240200.12682614870064973No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAAC239750.12658854767269268No Hit
CGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTCTACTCTTAGGA237760.12553782312683798No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAAGTGTTG237480.12538998248722025No Hit
AAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGG233700.1233941338523807No Hit
CTCTTATCGTTTTTTTACTTATTGGATGAAGTGGAAGCGGACCGCAAGGT232460.12273941101978783No Hit
TTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAAT232100.12254933019742217No Hit
GGAGTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGA228740.12077524252200925No Hit
GCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGG228380.12058516169964356No Hit
CGGGAATGCAGCCCAAAAATGGTGGTAAACTCCATCTAAAGCTAAATACT228100.12043732106002583No Hit
AAGGAGTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCA226310.11949219697104096No Hit
CCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAA226000.11932851626289276No Hit
GAAAGATGAAAAGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGT219080.1156747404551971No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGA215690.11388481271125371No Hit
CAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAAC215100.11357329136348776No Hit
CCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTG212330.1121107250358408No Hit
CTCAGCAATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG210400.11109168062704708No Hit
GGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTC205900.10871567034747619No Hit
ATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTA205460.10848334934236259No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG204350.10789726680673511No Hit
CCGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC200680.10595949842317398No Hit
GTTGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAG198410.10476093323770155No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA196330.10366268848625547No Hit
TAACAATACAGGGCTTTTACAAGTCTTGTAATTGGAATGAGTACAACTTA195850.10340924738976791No Hit
GTCCGACTTGCTTAATTGAAGCCGGACGACGAATGAGAGTGCCTAGTGGG195470.10320860652171526No Hit
ATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAG194850.10288124510541882No Hit
GAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCG194710.10280732478560996No Hit
ACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATC191300.10100683699597957No Hit
CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATA190480.10057387512281332No Hit
GGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCAAATCGATCGTC189650.10013563322680359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)