FastQCFastQC Report
Thu 20 Feb 2025
POR-245-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-245-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18939312
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1924591.0161879164354017No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1296520.6845655217042731No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA998170.5270360401687242No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC973850.5141950246133545No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC961810.5078378771097916No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC772570.40791872481957103No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC677170.35754730689266856No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG628750.33198143628448595No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG622830.32885566276113937No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA546260.28842652784853007No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA527410.2784736847885499No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC517350.27316198180799806No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA481970.25448126098772755No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA455000.24024103937883276No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT422230.2229384045207133No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA413160.21814942380166713No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC387910.20481736612185278No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC371500.19615284863568433No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA366330.1934230768255996No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG359640.1898907415433042No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC346610.18301087177823566No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC338070.1785017322698945No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA337320.17810573055663267No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT335870.17734012724432652No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC335260.17701804585087355No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT334950.17685436514272534No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG325450.17183834344140905No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC321790.16990585508069142No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT320580.16926697231662902No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC314920.1662784793872132No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT305110.16109877697774871No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC303150.16006389250042452No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA298560.15764036201526221No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG293730.15509011098185616No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG292520.15445122821779375No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG284700.15032225035418392No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC281800.1487910437295716No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT280160.1479251199832391No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA279780.14772447911518644No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC277870.1467159947520797No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG270220.14267677727680922No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA270120.1426239770483743No Hit
GGACGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTG268290.14165773286801547No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT266500.1407126087790306No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC263880.13932924279403602No Hit
GTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCC263670.13921836231432272No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT258210.13633546984177672No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT254890.13458250225773777No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT254530.1343924214353721No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC254400.1343237811384067No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT252540.1333416968895174No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC247240.1305432847824673No Hit
GTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTA245210.12947144014523865No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC243580.12861079642174963No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC240300.12687894892908466No Hit
CTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGC240270.12686310886055419No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC238560.12596022495431725No Hit
ATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGCACTTTGGGACGCAT236950.12511014127651524No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA234520.12382709572554694No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG232350.12268133076850944No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC232040.12251765006036124No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA231830.12240676958064792No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA228720.12076468247632227No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC228250.1205165214026782No Hit
GCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAAC226400.11953971717663239No Hit
GGGGTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCAT226100.11938131649132767No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT222350.1174013079250186No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCA222280.11736434776511417No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT219280.11578034091206692No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG216380.11424913428745459No Hit
GTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCAC215910.11400097321381052No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG215450.11375809216300993No Hit
GTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTG215100.11357329136348776No Hit
CGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGT214900.11346769090661793No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA214870.11345185083808747No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC214160.11307696921619961No Hit
GAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG212570.11223744558408458No Hit
GCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGC207530.10957631407096519No Hit
CTGCTGTTCACATGGAACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATT205400.10845166920530165No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA204840.10815598792606616No Hit
CTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAG203180.10727950413404669No Hit
GCCATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGC203070.10722142388276829No Hit
CCTATACCCAAATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCC202730.1070419031060896No Hit
CGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCC201860.10658254111870588No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT196520.1037630089202818No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT195360.10315052627043685No Hit
GCGGTTATGAGTACGACCGGACACGAAAATCATTGTCTCCCGCGGATTTT193690.1022687624555739No Hit
CTAGGCACTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCT193170.10199420126771237No Hit
CCATGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCT192790.10179356039965971No Hit
CTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTG191490.1011071574300059No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTACCTACATTGTTCTA191020.10085899635636182No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC190680.10067947557968315No Hit
CCTCACGGTACTTGTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGA190070.10035739418623021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)