FastQCFastQC Report
Thu 20 Feb 2025
POR-245-TP2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-245-TP2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16922913
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1870071.1050520675725273No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1491050.8810835344955091No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC898680.5310433256969411No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG815840.4820919424451334No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC808930.4780087210753846No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA783600.46304084881840374No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA668430.39498518960654116No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA609890.3603930363525476No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC556390.3287790937647673No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA547940.3237858635803422No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC535040.3161630624703915No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA514910.30426794724997996No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC425650.2515228908876386No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC423810.25043560762854483No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG416590.24616920266623127No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA403160.23823321670447634No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG402280.2377132116675185No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC397730.23502454926052035No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG395950.23397272089031007No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC374570.22133896215149246No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA369180.21815393130012545No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC365070.21572527141160627No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG358210.2116715957825937No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC355950.2103361283013155No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA345760.20431470633926913No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT344020.20328651456164784No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG342630.2024651429691803No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC337650.19952238719185048No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT336250.19869510645123567No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA325410.19228958985961814No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT325200.19216549774852593No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA319350.18870864608238547No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG312440.18462542471263665No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG302520.178763549750566No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC295610.17468032838081718No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC294550.17405395867720883No Hit
GATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGT292000.17254712589966043No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC291560.17228712338118146No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT291250.17210393978861677No Hit
GAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG290210.17148938838130293No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG289870.17128847734429647No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC287530.16990573667784026No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC287110.16965755245565584No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA285410.16865299727062355No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT283770.16768389697447478No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT283120.16729980234490363No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG265610.15695288393907125No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC261960.1547960448653255No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC260410.15388012690250194No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT257790.15233193008792278No Hit
CTTTATTTAAGTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTT247830.14644641853326315No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT247030.14597368668148328No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT245210.144898221718684No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT244200.1443013977558119No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC240640.14219774101539137No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA240330.14201455742282668No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT238560.14096863820076366No Hit
TTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCG232030.1371099644606103No Hit
CTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGC228560.13505949005351503No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC228220.13485857901650858No Hit
CTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAG221600.13094672294302995No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA219680.12981216649875824No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG212260.12542757857349973No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC211570.12501984735133956No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT209250.12364892498117788No Hit
CTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGC208810.12338892246269895No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC206730.12215981964807122No Hit
CGGCGCATTGCTGCTCCCACTTATTCTACACCTCTCATGTCTTTTCACAA204930.12109617298156647No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG203590.12030434712983515No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT202940.119920252500264No Hit
CTGCTGTTCACATGGAACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATT201020.11878569605599226No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTGCAACCATGGTAAGCCA200420.11843114716715733No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT198800.11747386516730304No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC198390.11723159009326585No Hit
CAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTG198210.11712522542661537No Hit
CCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACG197960.11697749672293417No Hit
CAACAGACACGCTCTTACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTC196250.11596703238975464No Hit
TGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAA195940.11578384879718995No Hit
GTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAA195710.11564793838980322No Hit
GCGGTTATGAGTACGACCGGACACGAAAATCATTGTCTCCCGCGGATTTT195160.11532293524170453No Hit
CAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCT194690.11504520527878385No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCCA194370.1148561125380719No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC192400.11369201035306392No Hit
TGCTTTCACAGTTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTTTT191420.11311291383463355No Hit
CGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCC190290.11244518009399446No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC189020.11169471827929389No Hit
AACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAG188590.11144062490896219No Hit
ATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTT187750.11094425646459331No Hit
CACAGACCTGTTATTGCCTTCCTGACTTTGGTTGGATACCAACAGTCCCT186630.11028243187210145No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT185560.10965015302034585No Hit
CTAGGCACTCTCATTCGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCT185130.10939605965001416No Hit
ATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGG184100.10878741739084755No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA183190.10824968490944792No Hit
TAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCT182310.10772967987249003No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC181150.1070442186874092No Hit
GTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTA180790.10683148935410824No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC180240.10650648620600957No Hit
TGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCA178820.10566738716910025No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA178530.10549602187283004No Hit
GTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCC178500.10547829442838831No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTACCTACATTGTTCTA175770.10386509698418941No Hit
CCCAGTCCCAACCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGG175060.10344554746573478No Hit
GGACGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTG174920.10336281939167329No Hit
CGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATG173560.10255917524364747No Hit
CCGCATTCGACAACCGGCCGGAAGGACGGGACCCTAGCCACAAGAGGCAT172200.10175553109562166No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT170440.1007155210217059No Hit
CTTCAAACGGTTTCACGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCA169680.100266425762515No Hit
GCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAAC169240.10000642324403605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)