FastQCFastQC Report
Thu 20 Feb 2025
POR-236-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-236-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19622258
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA2024001.031481697977878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1573240.8017629775329629No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG1163620.5930102437752067No Hit
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG1153340.5877712952301413No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG829490.4227291273002322No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT766590.3906736931091213No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG725900.36993703782714504No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT646590.32951865172703365No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT633290.32274063464051894No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC593280.30235052459304124No Hit
TGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGG574050.2925504292115617No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC543400.2769304123918868No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAA534100.27219089668477503No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC532350.27129905233128626No Hit
TTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGC484220.24677078448362058No Hit
GAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAAC433160.2207493143755423No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA421170.21463890649078202No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC405770.20679067618008082No Hit
AGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAA405590.2066989436180077No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA397870.20276463595576005No Hit
CGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTG397620.20263722961954733No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA393720.20064969077462952No Hit
CAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGA390280.198896579588343No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA374260.1907323815638343No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA369310.18820973610682318No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAG366070.18655854998950683No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA351130.1789447473374369No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT345630.1761418079407579No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA339240.17288530198716173No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT338250.1723807728957595No Hit
GACTAATCGAACTGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA336660.17157046859744685No Hit
GTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCT332470.16943513840252228No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA327870.1670908618162089No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT327230.16676470159550447No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA325580.16592381977650075No Hit
CGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCG321590.16389041465054635No Hit
AAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAG319540.16284568269360233No Hit
CGGGAATGCAGCCCAAAAATGGTGGTAAACTCCATCTAAAGCTAAATACT307570.1567454673157391No Hit
CAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAAC307440.1566792160209085No Hit
GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC301650.15372848527422278No Hit
TTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAAT298350.15204672163621535No Hit
GTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGA296900.1513077648861818No Hit
CTCTTATCGTTTTTTTACTTATTGGATGAAGTGGAAGCGGACCGCAAGGT294440.15005408653784902No Hit
ATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAG294160.14991139144129081No Hit
CTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG291710.14866280934640652No Hit
CTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCAT291250.14842838168777517No Hit
GCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGG290470.1480308739187916No Hit
TCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATG287720.14662940422045212No Hit
ACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGAT282720.14408127749619845No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG282130.14378059854273653No Hit
CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATA270950.13808298718730536No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGG265850.13548389792856663No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG264360.13472455616473905No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG263730.1344034921974831No Hit
CAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCG261600.13331799021295104No Hit
GTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCT255870.13039783698695634No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG250610.1277172076730415No Hit
CCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAA248710.1267489195178251No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAAC244170.1244352204522028No Hit
CGAACAACGAACTTAGAACTGGCAGTGACTAGGGGAATCCGACTGTTTAA243820.12425685158150505No Hit
GTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCT239140.12187180496760362No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC234090.11929819697610744No Hit
CCGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC232790.11863568402780149No Hit
GTTGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAGCAACTAG229590.11700488292427916No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACC229480.11694882413634557No Hit
CTCAAAGGTTCCGGGAAGGTAGGTCCGGACGCTCGCGTTCGGACTGTTAC228740.11657170138115604No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGA228050.11622005989320904No Hit
ACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATC225660.11500205531901578No Hit
GGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCAAATCGATCGTC220550.11239786980682856No Hit
CAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGC219110.11166400931024349No Hit
GGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTC217630.11090976379986442No Hit
CCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTG215510.10982935806878089No Hit
GCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGT215360.10975291426705326No Hit
CAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGG215110.10962550793084057No Hit
CCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATT211890.10798451432042122No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGACCTTGTGAAAAGACATGA210410.10723026881004215No Hit
GTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAA209860.10694997487037425No Hit
CTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCAAATCGATCGTCAAATT207740.1058695691392907No Hit
CTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAG207290.10564023773410787No Hit
CTGAAATCGGATTGTCCGGAGATAGGGTTTAATGCCTGGTAAAGCACCAC206720.10534975128754294No Hit
TTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAAGTGTTGT206590.10528349999271236No Hit
GTCCGACTTGCTTAATTGAAGCCGGACGACGAATGAGAGTGCCTAGTGGG206460.10521724869788177No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAA205290.10462098704440641No Hit
GTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTAT204850.10439675189267208No Hit
GTGAAATTCTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCA201800.10284239459087735No Hit
TGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATT199430.10163458252358112No Hit
CTCAGCAATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG197340.10056946555284312No Hit
GAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCG197290.10054398428560056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)