FastQCFastQC Report
Thu 20 Feb 2025
POR-216-TP4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-216-TP4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21522395
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1713680.796231088593997No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC1083020.5032060790632269No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC1029490.47833431177152913No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC879640.40870916085314857No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA815640.3789726933271135No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA810400.37653802004841935No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC693590.32226432049035436No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA677830.3149417153620682No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC676690.3144120345342607No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG555410.2580614285724242No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA535810.24895463539257598No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG530020.24626441434607996No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG520590.24188293170904074No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA505060.23466719201092628No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC498630.23167960628916995No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA494120.22958411459319464No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT466670.21682995781835615No Hit
GCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAAC454780.21130547971078498No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC431430.20045631538683312No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC428030.19887656554951252No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT413970.19234383533988667No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA412050.1914517413141056No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA404690.18803204754861158No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA396760.1843475133692138No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT394280.18319522525257992No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC376830.1750873915286844No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC364870.16953038915975663No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA363300.16880091644075856No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT360840.16765792097022658No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC357330.1660270615793456No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC349660.16246333179927233No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT345090.1603399621649914No Hit
GTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCAC344280.15996360999786502No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC342400.15909010126428771No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA341540.15869051748190663No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG327100.15198122699634498No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT318790.14812013254101136No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA317520.1475300495135416No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC315700.14668441871826995No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT314170.1459735312914757No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC310390.14421722117821925No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT306230.14228435078902696No Hit
CCTCACGGTACTTGTTCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGA305430.14191264494495154No Hit
CTGCATTCCCGAACAACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGG305390.14189405965274776No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC305170.14179184054562702No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG304570.14151306116257042No Hit
GTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTC302770.14067672301340067No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG297730.13833497619572543No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC293600.13641604477568597No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT293080.13617443597703693No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT291800.13557970662651625No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC290180.1348270022922635No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT286280.13301493630239572No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC283470.13170931952508075No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG280960.13054309243929405No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA274980.12776459125483014No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC268610.12480488347137947No Hit
GTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCC267940.12449357982696628No Hit
CTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGC264400.12284878146693247No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC262850.1221286013940363No Hit
GGACGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTG259200.12043269348044211No Hit
CTCTGATCCCTAGTTGGCATCGTTTATGGTTAGAACTAGGACGGTATCTG258940.12031188908111759No Hit
GTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTA254570.11828144590785553No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG254110.11806771504751212No Hit
GCCAAACTCCCAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAA254010.1180212518170027No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA252830.11747298569699142No Hit
CCCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAAT251350.11678532988545187No Hit
CTGATCCCTAGTTGGCATCGTTTATGGTTAGAACTAGGACGGTATCTGAT246420.11449469262133699No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG242370.11261293178570508No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC241410.11216688477281454No Hit
CTTCAAACGGTTTCACGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCA237700.11044309892091471No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT237670.11042915995176188No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG237670.11042915995176188No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC235200.10928151815817895No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG230450.10707451470898105No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCA229430.10660058975778484No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC229380.10657735814253015No Hit
GGGGTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCAT229300.10654018755812258No Hit
CTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC227990.10593151923844907No Hit
CAACAGACACGCTCTTACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTC227450.10568061779369815No Hit
CCAGAAACCAACAAAATAGAACCAAAGTCCTATTCCATTATTCCATGCTT225710.10487215758283405No Hit
CCCAGTCCCAACCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGG223820.10399400252620583No Hit
CCTATACCCAAATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCC223450.10382208857332095No Hit
CTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTG223300.10375239372755679No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT222800.10352007757500965No Hit
CTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA221150.10275343427160408No Hit
CGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAA219700.10207971742921733No Hit
GCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGC219590.10202860787565697No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT217100.10087167343597217No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT217070.10085773446681934No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)