FastQCFastQC Report
Thu 20 Feb 2025
POR-216-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-216-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16130742
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCA1286930.797812028733706No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC910940.5647229371097746No Hit
GTCGTCTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCA661910.41034070224419933No Hit
CCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGC629560.39028582814107377No Hit
CTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACC622460.3858842947212224No Hit
CCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCA506550.3140277118064377No Hit
CCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGC483890.2999800009199825No Hit
CTTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACAC431060.26722887267058143No Hit
GTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCG411000.2547929909237901No Hit
CTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCC400740.24843246516496267No Hit
CCCAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATA386550.23963559766810477No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA379540.23528985833385718No Hit
CAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCA375750.23294030739565483No Hit
CCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTG364230.22579866443837487No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA336140.2083847103871601No Hit
CTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAG313560.1943865942434638No Hit
ACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATC310390.19242140256164286No Hit
CTGGCTTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCACAGATCC310180.19229121636190077No Hit
GTCGGATTCCCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTC304530.18878858765455428No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCC302950.18780909148506622No Hit
CGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCC302700.18765410791394468No Hit
GCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCA301520.18692258545825108No Hit
CCTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGT299530.18568891623212372No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCATTCCAATTACA267950.16611139152805246No Hit
CACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCT267310.16571463358598135No Hit
CGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTC264160.16376184058985013No Hit
TTTTGTTCTACATGAGATTTCTGTTCTCATTGAGCTCACCTTAGGACACC253080.15689296871774405No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCAT251220.15573989094859989No Hit
CTCGTTTAAGATCAAAAATTGCAATGATCTATCCCCAGCACGACGATGTT242490.15032786464503617No Hit
CCGGCGTCTCCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAG235720.1461309095390652No Hit
CTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTT235170.14578994568259787No Hit
CAACTGTCTCTCTTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGA233100.14450668171371162No Hit
GCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAG230920.1431552249735319No Hit
CCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGC230420.14284525783128885No Hit
CCGCGTTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATAT224980.1394728153236844No Hit
GTCAGAATCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTAT221580.13736503875643166No Hit
CTACCACCAAGATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAA217650.13492869701840127No Hit
CGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAG217000.13452573973348528No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC214450.13294490730804573No Hit
AAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGG206830.12822100806026157No Hit
CTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGACAGT206540.12804122711776061No Hit
CCCAGCACGACGATGTTTAAAAGATTACCCACACCTCTCGGTGAAGGAGA205260.1272477112336184No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGC198140.1228337791280773No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA196810.12200926652971078No Hit
GTCGGATGGACCCATATGACCGCCAACGTCCTTAGCACATGGTACGAGAT194880.12081279336065259No Hit
CTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA194260.12042843410427122No Hit
CCGACGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGC193760.12011846696202816No Hit
GTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGT193420.11990768930530288No Hit
GTACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACT191750.11887239905021108No Hit
GCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTT190100.117849507480809No Hit
GTCCTTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCG185830.11520238808605332No Hit
CAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGG185590.11505360385777666No Hit
TACGACTTTTACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCAT185390.11492961700087943No Hit
CTCCTACTCGTCGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGC183360.11367115040337263No Hit
CTCACCTTAGGACACCTGCGTTATCATTTGACAGATGTGCCGCCCCAGCC182050.11285903649069584No Hit
GTAATTATCGTTGCTTTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTC179350.11118521392258335No Hit
CTGCGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC178900.1109062434945646No Hit
CTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGC177930.11030490723861308No Hit
GTCTGATGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCC176110.10917662684084836No Hit
CCCTAGTCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGA174430.1081351372429117No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT173740.10770738258661627No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCATCACTTGGCGAATTCTGC173280.10742221281575268No Hit
CGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTC172680.10705025224506103No Hit
TGAGCGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCG172440.10690146801678435No Hit
TCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCG172290.10680847787411143No Hit
GCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGCTCACCTC171970.10661009890307588No Hit
GGACGCATGAAGACCGAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTG170110.10545702113393172No Hit
CTCTAATCATTCGCTTTACCTGATAAAACTGTACACTGAGTTCCAGCTAT166410.10316326428133311No Hit
GTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTA165860.10282230042486576No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG164620.10205358191210298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)