FastQCFastQC Report
Fri 21 Feb 2025
ACR-229-TP4_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameACR-229-TP4_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22524538
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAACTGGACGGGAAGTTCGAAAGAGCGAAGAAGAGGCTTTAAACTCG395820.17572835456158967No Hit
GAAGACCCCCGAAGAGCATCACCCCAACCCCAAAGCAACGCCGTTGCCTG274520.12187597366036987No Hit
ATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAGGAGGATGGCCTGGCT263530.11699685027945968No Hit
GATTAAACTGGACGGGAAGTTCGAAAGAGCGAAGAAGAGGCTTTAAACTC227850.101156347801673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)