FastQCFastQC Report
Tue 31 Dec 2024
1A8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54274968
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACAAAAAAAATTACTTATCTCAAAAAAATCAACAAATTTTAATAAT1750870.32259254395138476No Hit
CATACTTTTTACAACATTTTACTAAAAATTAAATCAAAACCCTAATACAC707930.1304339783304893No Hit
CCTAATACACAATACTTTACAAAAAAAATTACTTATCTCAAAAAAATCAA603960.11127781779622606No Hit
AATTTTAACCCAATTTTTAACAAAATATTACAAAAAATATAACATTTAAT579260.10672691690946737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)