FastQCFastQC Report
Thu 20 Feb 2025
ACR-225-TP3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameACR-225-TP3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21126403
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATCATAATCTGGGGG952730.45096649912434217TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT591520.2799908720855131No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATCATAATCTAGGGG288200.13641697547850432TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)