Bismark report for: ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-57-TP2_S12_R1_001.fastq.gz and ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-57-TP2_S12_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/F-Ptuh/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 241592172 Number of paired-end alignments with a unique best hit: 141174678 Mapping efficiency: 58.4% Sequence pairs with no alignments under any condition: 71647653 Sequence pairs did not map uniquely: 28769841 Sequence pairs which were discarded because genomic sequence could not be extracted: 232 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 43695068 ((converted) top strand) GA/CT/CT: 26967206 (complementary to (converted) top strand) GA/CT/GA: 26927590 (complementary to (converted) bottom strand) CT/GA/GA: 43584582 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5592250056 Total methylated C's in CpG context: 36067872 Total methylated C's in CHG context: 18974329 Total methylated C's in CHH context: 97064058 Total methylated C's in Unknown context: 17354214 Total unmethylated C's in CpG context: 864973038 Total unmethylated C's in CHG context: 1061369484 Total unmethylated C's in CHH context: 3513801275 Total unmethylated C's in Unknown context: 64120788 C methylated in CpG context: 4.0% C methylated in CHG context: 1.8% C methylated in CHH context: 2.7% C methylated in unknown context (CN or CHN): 21.3% Bismark completed in 0d 18h 26m 20s