Bismark

Bismark Processing Report

../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-57-TP2_S12_R1_001.fastq.gz and ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-57-TP2_S12_R2_001.fastq.gz

Data processed at 06:14:03 on 2025-05-01


Alignment Stats

Sequence pairs analysed in total 241592172
Paired-end alignments with a unique best hit 141174678
Pairs without alignments under any condition 71647653
Pairs that did not map uniquely 28769841
Genomic sequence context not extractable (edges of chromosomes) 232

Cytosine Methylation

Total C's analysed 5592250056
Methylated C's in CpG context 36067872
Methylated C's in CHG context 18974329
Methylated C's in CHH context 97064058
Methylated C's in Unknown context 17354214
Unmethylated C's in CpG context 864973038
Unmethylated C's in CHG context 1061369484
Unmethylated C's in CHH context 3513801275
Unmethylated C's in Unknown context 64120788
Percentage methylation (CpG context) 4.0%
Percentage methylation (CHG context) 1.8%
Percentage methylation (CHH context) 2.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 43695068 original top strand
CTOT 26967206 complementary to original top strand
CTOB 26927590 complementary to original bottom strand
OB 43584582 original bottom strand

Deduplication

Alignments analysed 141174446
Unique alignments 91172395
Duplicates removed 50002051
Duplicated alignments were found at 29739029 different positions

Cytosine Methylation after Extraction

Total C's analysed 2027773994
Methylated C's in CpG context 13006511
Methylated C's in CHG context 7144626
Methylated C's in CHH context 38058783
Unmethylated C's in CpG context 308566363
Unmethylated C's in CHG context 378910774
Unmethylated C's in CHH context 1282086937
Percentage methylation (CpG context) 4.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 2578794884 116613941 30.33 30.97 22.114
T 2472421423 116748546 29.08 31.00 21.177
C 1619338570 71613560 19.05 19.02 22.612
G 1831816171 71603867 21.54 19.01 25.583
AC 462720023 20840071 5.50 5.53 22.203
CA 553889223 24943498 6.58 6.62 22.206
TC 487495050 22924241 5.79 6.09 21.265
CT 480400270 22427370 5.71 5.96 21.420
CC 304580407 13853764 3.62 3.68 21.985
CG 263526296 10388885 3.13 2.76 25.366
GC 348102854 13995432 4.13 3.72 24.873
GG 385036388 13850871 4.57 3.68 27.799
AG 562580911 22410051 6.68 5.95 25.104
GA 576215654 22896723 6.84 6.08 25.166
TG 600831398 24954009 7.14 6.63 24.078
GT 496944037 20860801 5.90 5.54 23.822
TT 834443704 42681247 9.91 11.33 19.551
TA 527603301 26188985 6.27 6.95 20.146
AT 635515504 30779082 7.55 8.17 20.648
AA 900298759 42584672 10.69 11.31 21.141

M-Bias Plot