Bismark report for: ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-53-TP2_S15_R1_001.fastq.gz and ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-53-TP2_S15_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/F-Ptuh/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 141417553 Number of paired-end alignments with a unique best hit: 78836445 Mapping efficiency: 55.7% Sequence pairs with no alignments under any condition: 42392655 Sequence pairs did not map uniquely: 20188453 Sequence pairs which were discarded because genomic sequence could not be extracted: 93 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 22064650 ((converted) top strand) GA/CT/CT: 18425000 (complementary to (converted) top strand) GA/CT/GA: 18416810 (complementary to (converted) bottom strand) CT/GA/GA: 19929892 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3026230854 Total methylated C's in CpG context: 22895388 Total methylated C's in CHG context: 16320787 Total methylated C's in CHH context: 58134446 Total methylated C's in Unknown context: 7696769 Total unmethylated C's in CpG context: 533555765 Total unmethylated C's in CHG context: 606361912 Total unmethylated C's in CHH context: 1788962556 Total unmethylated C's in Unknown context: 36081212 C methylated in CpG context: 4.1% C methylated in CHG context: 2.6% C methylated in CHH context: 3.1% C methylated in unknown context (CN or CHN): 17.6% Bismark completed in 0d 10h 14m 21s