Bismark report for: ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-50-TP2_S14_R1_001.fastq.gz and ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-50-TP2_S14_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/F-Ptuh/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 167114293 Number of paired-end alignments with a unique best hit: 95287182 Mapping efficiency: 57.0% Sequence pairs with no alignments under any condition: 44781799 Sequence pairs did not map uniquely: 27045312 Sequence pairs which were discarded because genomic sequence could not be extracted: 87 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 27818348 ((converted) top strand) GA/CT/CT: 21421024 (complementary to (converted) top strand) GA/CT/GA: 21393822 (complementary to (converted) bottom strand) CT/GA/GA: 24653901 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3215206295 Total methylated C's in CpG context: 24480584 Total methylated C's in CHG context: 18188843 Total methylated C's in CHH context: 65242926 Total methylated C's in Unknown context: 6833414 Total unmethylated C's in CpG context: 553212268 Total unmethylated C's in CHG context: 643381408 Total unmethylated C's in CHH context: 1910700266 Total unmethylated C's in Unknown context: 35089127 C methylated in CpG context: 4.2% C methylated in CHG context: 2.7% C methylated in CHH context: 3.3% C methylated in unknown context (CN or CHN): 16.3% Bismark completed in 0d 11h 26m 2s