Bismark

Bismark Processing Report

../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-50-TP2_S14_R1_001.fastq.gz and ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-50-TP2_S14_R2_001.fastq.gz

Data processed at 06:14:03 on 2025-05-01


Alignment Stats

Sequence pairs analysed in total 167114293
Paired-end alignments with a unique best hit 95287182
Pairs without alignments under any condition 44781799
Pairs that did not map uniquely 27045312
Genomic sequence context not extractable (edges of chromosomes) 87

Cytosine Methylation

Total C's analysed 3215206295
Methylated C's in CpG context 24480584
Methylated C's in CHG context 18188843
Methylated C's in CHH context 65242926
Methylated C's in Unknown context 6833414
Unmethylated C's in CpG context 553212268
Unmethylated C's in CHG context 643381408
Unmethylated C's in CHH context 1910700266
Unmethylated C's in Unknown context 35089127
Percentage methylation (CpG context) 4.2%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 3.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 27818348 original top strand
CTOT 21421024 complementary to original top strand
CTOB 21393822 complementary to original bottom strand
OB 24653901 original bottom strand

Deduplication

Alignments analysed 95287095
Unique alignments 75134038
Duplicates removed 20153057
Duplicated alignments were found at 12141545 different positions

Cytosine Methylation after Extraction

Total C's analysed 1354399842
Methylated C's in CpG context 8691199
Methylated C's in CHG context 4652695
Methylated C's in CHH context 24678856
Unmethylated C's in CpG context 231324950
Unmethylated C's in CHG context 269968140
Unmethylated C's in CHH context 815084002
Percentage methylation (CpG context) 3.6%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 1956198216 116613941 28.75 30.97 16.775
T 1986016160 116748546 29.19 31.00 17.011
C 1287567318 71613560 18.93 19.02 17.979
G 1573348489 71603867 23.13 19.01 21.973
AC 345827378 20840071 5.14 5.53 16.594
CA 414393283 24943498 6.16 6.62 16.613
TC 388751997 22924241 5.78 6.09 16.958
CT 385881306 22427370 5.74 5.96 17.206
CC 240174640 13853764 3.57 3.68 17.336
CG 231362813 10388885 3.44 2.76 22.270
GC 297462727 13995432 4.42 3.72 21.254
GG 341843772 13850871 5.08 3.68 24.680
AG 460848273 22410051 6.85 5.95 20.564
GA 478491040 22896723 7.11 6.08 20.898
TG 518986611 24954009 7.71 6.63 20.798
GT 431879504 20860801 6.42 5.54 20.703
TT 666459683 42681247 9.91 11.33 15.615
TA 390526715 26188985 5.80 6.95 14.912
AT 476203463 30779082 7.08 8.17 15.472
AA 658456465 42584672 9.79 11.31 15.462

M-Bias Plot