Bismark report for: ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-48-TP2_S11_R1_001.fastq.gz and ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-48-TP2_S11_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/F-Ptuh/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 177078360 Number of paired-end alignments with a unique best hit: 104251182 Mapping efficiency: 58.9% Sequence pairs with no alignments under any condition: 50943986 Sequence pairs did not map uniquely: 21883192 Sequence pairs which were discarded because genomic sequence could not be extracted: 128 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 30528263 ((converted) top strand) GA/CT/CT: 21610628 (complementary to (converted) top strand) GA/CT/GA: 21607059 (complementary to (converted) bottom strand) CT/GA/GA: 30505104 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4027104417 Total methylated C's in CpG context: 26211877 Total methylated C's in CHG context: 14132535 Total methylated C's in CHH context: 73475503 Total methylated C's in Unknown context: 12765557 Total unmethylated C's in CpG context: 639104269 Total unmethylated C's in CHG context: 772862587 Total unmethylated C's in CHH context: 2501317646 Total unmethylated C's in Unknown context: 47337161 C methylated in CpG context: 3.9% C methylated in CHG context: 1.8% C methylated in CHH context: 2.9% C methylated in unknown context (CN or CHN): 21.2% Bismark completed in 0d 14h 42m 1s