Bismark report for: ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-47-TP2_S13_R1_001.fastq.gz and ../output/01.00-F-Ptuh-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POC-47-TP2_S13_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/F-Ptuh/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 151987190 Number of paired-end alignments with a unique best hit: 90000133 Mapping efficiency: 59.2% Sequence pairs with no alignments under any condition: 38018588 Sequence pairs did not map uniquely: 23968469 Sequence pairs which were discarded because genomic sequence could not be extracted: 93 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25028895 ((converted) top strand) GA/CT/CT: 20880873 (complementary to (converted) top strand) GA/CT/GA: 20847563 (complementary to (converted) bottom strand) CT/GA/GA: 23242709 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3022744266 Total methylated C's in CpG context: 21446654 Total methylated C's in CHG context: 15194531 Total methylated C's in CHH context: 62636135 Total methylated C's in Unknown context: 7115726 Total unmethylated C's in CpG context: 516406005 Total unmethylated C's in CHG context: 602797818 Total unmethylated C's in CHH context: 1804263123 Total unmethylated C's in Unknown context: 35351450 C methylated in CpG context: 4.0% C methylated in CHG context: 2.5% C methylated in CHH context: 3.4% C methylated in unknown context (CN or CHN): 16.8% Bismark completed in 0d 11h 8m 24s