FastQCFastQC Report
Fri 11 Apr 2025
trimmed_POC-53-TP2_S15_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_POC-53-TP2_S15_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141417553
Total Bases15.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATGATACGGCGACCACCG5041820.3565200990290081No Hit
TCCTAATGATACGGCGACCACCG3678700.2601303672677747No Hit
CCCTAATGATACGGCGACCACCG3102130.21935961513914753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2632250.1861331881481502No Hit
ACCTAATGATACGGCGACCACCG2534880.17924790425414872No Hit
CTTAATGATACGGCGACCACCG2033150.14376928159688918No Hit
CTCTAATGATACGGCGACCACCG1929480.13643850845022046No Hit
ACTAATGATACGGCGACCACCG1910330.13508436254727163No Hit
GCCTAATGATACGGCGACCACCG1735910.12275067438057No Hit
TCTAATGATACGGCGACCACCG1643790.11623663153045788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)