FastQCFastQC Report
Fri 11 Apr 2025
trimmed_POC-53-TP2_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_POC-53-TP2_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141417553
Total Bases14.8 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGG7911500.5594425749963302No Hit
CGGTGGTCGCCGTATCATTAGGA5632870.39831476931297205No Hit
CGGTGGTCGCCGTATCATTAGGG4834290.3418451173455109No Hit
CGGTGGTCGCCGTATCATTAGGT3924000.27747616308988177No Hit
CGGTGGTCGCCGTATCATTAAG3368570.23820027489798243No Hit
CGGTGGTCGCCGTATCATTAGAG2890510.20439541900431554No Hit
CGGTGGTCGCCGTATCATTAGGC2857990.20209584590959512No Hit
CGGTGGTCGCCGTATCATTAGT2847270.2013378070542629No Hit
CGGTGGTCGCCGTATCATTAGA2466960.17444510583491712No Hit
CGGTGGTCGCCGTATCATTA2164000.153022022662208No Hit
CGGTGGTCGCCGTATCATTAG1976800.13978462772580996No Hit
CGGTGGTCGCCGTATCATTAAGG1829570.12937361460355631No Hit
CGGTGGTCGCCGTATCATTACGG1535190.10855724536543211No Hit
CGGTGGTCGCCGTATCATTAGC1423860.10068481385758386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)