FastQCFastQC Report
Fri 11 Apr 2025
trimmed_POC-50-TP2_S14_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_POC-50-TP2_S14_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167114293
Total Bases16 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATGATACGGCGACCACCG6986390.41806059042478194No Hit
TCCTAATGATACGGCGACCACCG5222790.3125280253556768No Hit
CCCTAATGATACGGCGACCACCG3797330.22722951650820197No Hit
ACCTAATGATACGGCGACCACCG3313470.19827567950755715No Hit
GCCTAATGATACGGCGACCACCG2748430.16446408925656644No Hit
CTTAATGATACGGCGACCACCG2606960.1559986254437255No Hit
ACTAATGATACGGCGACCACCG2589200.15493587972155082No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2470280.14781979181158372No Hit
CTCTAATGATACGGCGACCACCG2462300.14734227430803898No Hit
TCTAATGATACGGCGACCACCG2447740.14647101430157144No Hit
TAATGATACGGCGACCACCG2375560.14215181462665197No Hit
CTAATGATACGGCGACCACCG1933510.11569985818029342No Hit
GCTAATGATACGGCGACCACCG1676180.10030141467313033No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)