FastQCFastQC Report
Fri 11 Apr 2025
trimmed_POC-50-TP2_S14_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_POC-50-TP2_S14_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167114293
Total Bases14.8 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGG13155850.7872366728081122No Hit
CGGTGGTCGCCGTATCATTAGGA9705560.5807737821683511No Hit
CGGTGGTCGCCGTATCATTAGGG7368570.4409299688088319No Hit
CGGTGGTCGCCGTATCATTAGGT6301610.37708384404917417No Hit
CGGTGGTCGCCGTATCATTAGGC5501920.32923096530109486No Hit
CGGTGGTCGCCGTATCATTAAG5129540.30694801192139803No Hit
CGGTGGTCGCCGTATCATTAGT4569070.2734098872081516No Hit
CGGTGGTCGCCGTATCATTAGAG4495170.2689877639610395No Hit
CGGTGGTCGCCGTATCATTAGA4350160.26031046907519756No Hit
CGGTGGTCGCCGTATCATTA4313600.25812274477324326No Hit
CGGTGGTCGCCGTATCATTAG3367550.20151178810300804No Hit
CGGTGGTCGCCGTATCATTAGC2972040.17784475203446543No Hit
CGGTGGTCGCCGTATCATTACGG2745230.16427260354085932No Hit
CGGTGGTCGCCGTATCATTAC2621170.15684894170003758No Hit
CGGTGGTCGCCGTATCATTAGAC2512440.15034261611602545No Hit
CGGTGGTCGCCGTATCATTAAGG2438850.1459390430476225No Hit
CGGTGGTCGCCGTATCATTAGAA2162760.12941801453212623No Hit
CGGTGGTCGCCGTATCATTAAC2035930.1218285978686455No Hit
CGGTGGTCGCCGTATCATTAGAT1979100.11842793123625878No Hit
CGGTGGTCGCCGTATCATTAGTA1851250.11077747850089638No Hit
CGGTGGTCGCCGTATCATTAAGA1804460.10797759830154086No Hit
CGGTGGTCGCCGTATCATTACAG1761790.10542425596115827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)