FastQCFastQC Report
Fri 11 Apr 2025
trimmed_POC-47-TP2_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_POC-47-TP2_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151987190
Total Bases14.4 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATGATACGGCGACCACCG4272450.2811059274140143No Hit
TCCTAATGATACGGCGACCACCG2904500.1911016316572469No Hit
CCCTAATGATACGGCGACCACCG2456460.16162283150310233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2079350.13681087202151707No Hit
ACCTAATGATACGGCGACCACCG1807050.1188948884442169No Hit
CTTAATGATACGGCGACCACCG1639370.1078623797176591No Hit
GCCTAATGATACGGCGACCACCG1628890.10717284792224922No Hit
CTCTAATGATACGGCGACCACCG1526490.10043543801290095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)