Bismark report for: ../output/01.00-E-Peve-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POR-79-TP2_S10_R1_001.fastq.gz and ../output/01.00-E-Peve-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POR-79-TP2_S10_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/E-Peve/data/ with the specified options: -q --score-min L,0,-1.0 -p 48 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 267531009 Number of paired-end alignments with a unique best hit: 147058074 Mapping efficiency: 55.0% Sequence pairs with no alignments under any condition: 79040089 Sequence pairs did not map uniquely: 41432846 Sequence pairs which were discarded because genomic sequence could not be extracted: 19080 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 38049264 ((converted) top strand) GA/CT/CT: 35288060 (complementary to (converted) top strand) GA/CT/GA: 35314264 (complementary to (converted) bottom strand) CT/GA/GA: 38387406 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 6007547193 Total methylated C's in CpG context: 81514938 Total methylated C's in CHG context: 22267955 Total methylated C's in CHH context: 104025447 Total methylated C's in Unknown context: 14712308 Total unmethylated C's in CpG context: 1013411203 Total unmethylated C's in CHG context: 1141944448 Total unmethylated C's in CHH context: 3644383202 Total unmethylated C's in Unknown context: 73888265 C methylated in CpG context: 7.4% C methylated in CHG context: 1.9% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 16.6% Bismark completed in 1d 17h 14m 11s