Bismark report for: ../output/01.00-E-Peve-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POR-76-TP2_S6_R1_001.fastq.gz and ../output/01.00-E-Peve-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POR-76-TP2_S6_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/E-Peve/data/ with the specified options: -q --score-min L,0,-1.0 -p 48 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 173500998 Number of paired-end alignments with a unique best hit: 98546250 Mapping efficiency: 56.8% Sequence pairs with no alignments under any condition: 43820661 Sequence pairs did not map uniquely: 31134087 Sequence pairs which were discarded because genomic sequence could not be extracted: 11761 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 28826966 ((converted) top strand) GA/CT/CT: 20227940 (complementary to (converted) top strand) GA/CT/GA: 20261300 (complementary to (converted) bottom strand) CT/GA/GA: 29218283 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3847749515 Total methylated C's in CpG context: 47480036 Total methylated C's in CHG context: 17830772 Total methylated C's in CHH context: 74535138 Total methylated C's in Unknown context: 10391621 Total unmethylated C's in CpG context: 676604903 Total unmethylated C's in CHG context: 737281551 Total unmethylated C's in CHH context: 2294017115 Total unmethylated C's in Unknown context: 38399470 C methylated in CpG context: 6.6% C methylated in CHG context: 2.4% C methylated in CHH context: 3.1% C methylated in unknown context (CN or CHN): 21.3% Bismark completed in 1d 6h 27m 21s