Bismark report for: ../output/01.00-E-Peve-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POR-73-TP2_S7_R1_001.fastq.gz and ../output/01.00-E-Peve-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POR-73-TP2_S7_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/E-Peve/data/ with the specified options: -q --score-min L,0,-1.0 -p 48 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 102561868 Number of paired-end alignments with a unique best hit: 61680314 Mapping efficiency: 60.1% Sequence pairs with no alignments under any condition: 29688557 Sequence pairs did not map uniquely: 11192997 Sequence pairs which were discarded because genomic sequence could not be extracted: 11356 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 18614980 ((converted) top strand) GA/CT/CT: 12216738 (complementary to (converted) top strand) GA/CT/GA: 12220945 (complementary to (converted) bottom strand) CT/GA/GA: 18616295 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2962310396 Total methylated C's in CpG context: 38310421 Total methylated C's in CHG context: 9626500 Total methylated C's in CHH context: 43218935 Total methylated C's in Unknown context: 7482071 Total unmethylated C's in CpG context: 463611232 Total unmethylated C's in CHG context: 549194379 Total unmethylated C's in CHH context: 1858348929 Total unmethylated C's in Unknown context: 24226368 C methylated in CpG context: 7.6% C methylated in CHG context: 1.7% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 23.6% Bismark completed in 0d 21h 54m 38s