Bismark report for: ../output/01.00-E-Peve-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POR-71-TP2_S9_R1_001.fastq.gz and ../output/01.00-E-Peve-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_POR-71-TP2_S9_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/E-Peve/data/ with the specified options: -q --score-min L,0,-1.0 -p 48 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 236709796 Number of paired-end alignments with a unique best hit: 132052904 Mapping efficiency: 55.8% Sequence pairs with no alignments under any condition: 66375041 Sequence pairs did not map uniquely: 38281851 Sequence pairs which were discarded because genomic sequence could not be extracted: 17665 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 34407731 ((converted) top strand) GA/CT/CT: 31419253 (complementary to (converted) top strand) GA/CT/GA: 31482943 (complementary to (converted) bottom strand) CT/GA/GA: 34725312 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5285849436 Total methylated C's in CpG context: 69970819 Total methylated C's in CHG context: 19181560 Total methylated C's in CHH context: 91424567 Total methylated C's in Unknown context: 11983188 Total unmethylated C's in CpG context: 900501437 Total unmethylated C's in CHG context: 1011829797 Total unmethylated C's in CHH context: 3192941256 Total unmethylated C's in Unknown context: 62425874 C methylated in CpG context: 7.2% C methylated in CHG context: 1.9% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 16.1% Bismark completed in 1d 19h 26m 56s