diamond v2.1.8.162 (C) Max Planck Society for the Advancement of Science, Benjamin Buchfink, University of Tuebingen Documentation, support and updates available at http://www.diamondsearch.org Please cite: http://dx.doi.org/10.1038/s41592-021-01101-x Nature Methods (2021) #CPU threads: 40 Scoring parameters: (Matrix=BLOSUM62 Lambda=0.267 K=0.041 Penalties=11/1) Temporary directory: ../output/30.00-Apul-transcriptome-GOslims #Target sequences to report alignments for: 1 Opening the database... [0.08s] Database: ../../M-multi-species/data/blastdbs/20250618-diamond.dmnd (type: Diamond database, sequences: 573661, letters: 207922125) Block size = 15000000000 Opening the input file... [0.036s] Opening the output file... [0s] Loading query sequences... [2.042s] Masking queries... [0.854s] Algorithm: Double-indexed Building query histograms... [1.264s] Seeking in database... [0s] Loading reference sequences... [0.58s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 1/4. Building reference seed array... [0.233s] Building query seed array... [0.149s] Computing hash join... [0.075s] Masking low complexity seeds... [0.017s] Searching alignments... [0.256s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 2/4. Building reference seed array... [0.156s] Building query seed array... [0.121s] Computing hash join... [0.074s] Masking low complexity seeds... [0.018s] Searching alignments... [0.252s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 3/4. Building reference seed array... [0.164s] Building query seed array... [0.126s] Computing hash join... [0.073s] Masking low complexity seeds... [0.019s] Searching alignments... [0.251s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 4/4. Building reference seed array... [0.133s] Building query seed array... [0.111s] Computing hash join... [0.075s] Masking low complexity seeds... [0.018s] Searching alignments... [0.245s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 1/4. Building reference seed array... [0.133s] Building query seed array... [0.107s] Computing hash join... [0.072s] Masking low complexity seeds... [0.019s] Searching alignments... [0.226s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 2/4. Building reference seed array... [0.154s] Building query seed array... [0.122s] Computing hash join... [0.071s] Masking low complexity seeds... [0.018s] Searching alignments... [0.219s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 3/4. Building reference seed array... [0.159s] Building query seed array... [0.126s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.228s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 4/4. Building reference seed array... [0.133s] Building query seed array... [0.107s] Computing hash join... [0.074s] Masking low complexity seeds... [0.018s] Searching alignments... [0.222s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 1/4. Building reference seed array... [0.133s] Building query seed array... [0.11s] Computing hash join... [0.074s] Masking low complexity seeds... [0.018s] Searching alignments... [0.24s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 2/4. Building reference seed array... [0.158s] Building query seed array... [0.125s] Computing hash join... [0.075s] Masking low complexity seeds... [0.017s] Searching alignments... [0.241s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 3/4. Building reference seed array... [0.163s] Building query seed array... [0.124s] Computing hash join... [0.075s] Masking low complexity seeds... [0.018s] Searching alignments... [0.239s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 4/4. Building reference seed array... [0.134s] Building query seed array... [0.107s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.239s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 1/4. Building reference seed array... [0.133s] Building query seed array... [0.107s] Computing hash join... [0.073s] Masking low complexity seeds... [0.017s] Searching alignments... [0.232s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 2/4. Building reference seed array... [0.157s] Building query seed array... [0.12s] Computing hash join... [0.073s] Masking low complexity seeds... [0.019s] Searching alignments... [0.241s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 3/4. Building reference seed array... [0.161s] Building query seed array... [0.125s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.232s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 4/4. Building reference seed array... [0.128s] Building query seed array... [0.11s] Computing hash join... [0.073s] Masking low complexity seeds... [0.017s] Searching alignments... [0.237s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 1/4. Building reference seed array... [0.13s] Building query seed array... [0.108s] Computing hash join... [0.071s] Masking low complexity seeds... [0.019s] Searching alignments... [0.227s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 2/4. Building reference seed array... [0.156s] Building query seed array... [0.123s] Computing hash join... [0.073s] Masking low complexity seeds... [0.016s] Searching alignments... [0.23s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 3/4. Building reference seed array... [0.166s] Building query seed array... [0.125s] Computing hash join... [0.072s] Masking low complexity seeds... [0.019s] Searching alignments... [0.23s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 4/4. Building reference seed array... [0.133s] Building query seed array... [0.11s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.231s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 1/4. Building reference seed array... [0.134s] Building query seed array... [0.108s] Computing hash join... [0.073s] Masking low complexity seeds... [0.017s] Searching alignments... [0.232s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 2/4. Building reference seed array... [0.162s] Building query seed array... [0.125s] Computing hash join... [0.072s] Masking low complexity seeds... [0.017s] Searching alignments... [0.231s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 3/4. Building reference seed array... [0.165s] Building query seed array... [0.135s] Computing hash join... [0.073s] Masking low complexity seeds... [0.021s] Searching alignments... [0.24s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 4/4. Building reference seed array... [0.134s] Building query seed array... [0.109s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.237s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 1/4. Building reference seed array... [0.131s] Building query seed array... [0.112s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.224s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 2/4. Building reference seed array... [0.159s] Building query seed array... [0.118s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.224s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 3/4. Building reference seed array... [0.164s] Building query seed array... [0.125s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.224s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 4/4. Building reference seed array... [0.13s] Building query seed array... [0.106s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.215s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 1/4. Building reference seed array... [0.124s] Building query seed array... [0.109s] Computing hash join... [0.073s] Masking low complexity seeds... [0.02s] Searching alignments... [0.221s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 2/4. Building reference seed array... [0.154s] Building query seed array... [0.122s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.228s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 3/4. Building reference seed array... [0.158s] Building query seed array... [0.125s] Computing hash join... [0.072s] Masking low complexity seeds... [0.017s] Searching alignments... [0.236s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 4/4. Building reference seed array... [0.132s] Building query seed array... [0.11s] Computing hash join... [0.072s] Masking low complexity seeds... [0.017s] Searching alignments... [0.243s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 1/4. Building reference seed array... [0.135s] Building query seed array... [0.114s] Computing hash join... [0.074s] Masking low complexity seeds... [0.017s] Searching alignments... [0.256s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 2/4. Building reference seed array... [0.162s] Building query seed array... [0.125s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.25s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 3/4. Building reference seed array... [0.166s] Building query seed array... [0.126s] Computing hash join... [0.075s] Masking low complexity seeds... [0.017s] Searching alignments... [0.251s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 4/4. Building reference seed array... [0.137s] Building query seed array... [0.109s] Computing hash join... [0.074s] Masking low complexity seeds... [0.018s] Searching alignments... [0.243s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 1/4. Building reference seed array... [0.127s] Building query seed array... [0.111s] Computing hash join... [0.074s] Masking low complexity seeds... [0.018s] Searching alignments... [0.232s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 2/4. Building reference seed array... [0.158s] Building query seed array... [0.121s] Computing hash join... [0.074s] Masking low complexity seeds... [0.017s] Searching alignments... [0.226s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 3/4. Building reference seed array... [0.162s] Building query seed array... [0.122s] Computing hash join... [0.072s] Masking low complexity seeds... [0.019s] Searching alignments... [0.236s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 4/4. Building reference seed array... [0.13s] Building query seed array... [0.106s] Computing hash join... [0.073s] Masking low complexity seeds... [0.017s] Searching alignments... [0.226s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 1/4. Building reference seed array... [0.132s] Building query seed array... [0.109s] Computing hash join... [0.072s] Masking low complexity seeds... [0.017s] Searching alignments... [0.246s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 2/4. Building reference seed array... [0.16s] Building query seed array... [0.122s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.236s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 3/4. Building reference seed array... [0.163s] Building query seed array... [0.126s] Computing hash join... [0.072s] Masking low complexity seeds... [0.017s] Searching alignments... [0.247s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 4/4. Building reference seed array... [0.134s] Building query seed array... [0.11s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.244s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 1/4. Building reference seed array... [0.13s] Building query seed array... [0.108s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.224s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 2/4. Building reference seed array... [0.159s] Building query seed array... [0.122s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.233s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 3/4. Building reference seed array... [0.16s] Building query seed array... [0.125s] Computing hash join... [0.073s] Masking low complexity seeds... [0.017s] Searching alignments... [0.23s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 4/4. Building reference seed array... [0.132s] Building query seed array... [0.107s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.229s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 1/4. Building reference seed array... [0.136s] Building query seed array... [0.11s] Computing hash join... [0.073s] Masking low complexity seeds... [0.018s] Searching alignments... [0.231s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 2/4. Building reference seed array... [0.158s] Building query seed array... [0.121s] Computing hash join... [0.073s] Masking low complexity seeds... [0.016s] Searching alignments... [0.228s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 3/4. Building reference seed array... [0.163s] Building query seed array... [0.122s] Computing hash join... [0.074s] Masking low complexity seeds... [0.016s] Searching alignments... [0.224s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 4/4. Building reference seed array... [0.131s] Building query seed array... [0.11s] Computing hash join... [0.073s] Masking low complexity seeds... [0.017s] Searching alignments... [0.223s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 1/4. Building reference seed array... [0.132s] Building query seed array... [0.114s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.229s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 2/4. Building reference seed array... [0.159s] Building query seed array... [0.126s] Computing hash join... [0.071s] Masking low complexity seeds... [0.016s] Searching alignments... [0.24s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 3/4. Building reference seed array... [0.163s] Building query seed array... [0.125s] Computing hash join... [0.073s] Masking low complexity seeds... [0.017s] Searching alignments... [0.236s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 4/4. Building reference seed array... [0.129s] Building query seed array... [0.109s] Computing hash join... [0.072s] Masking low complexity seeds... [0.016s] Searching alignments... [0.232s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 1/4. Building reference seed array... [0.134s] Building query seed array... [0.11s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.234s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 2/4. Building reference seed array... [0.16s] Building query seed array... [0.122s] Computing hash join... [0.074s] Masking low complexity seeds... [0.017s] Searching alignments... [0.231s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 3/4. Building reference seed array... [0.161s] Building query seed array... [0.124s] Computing hash join... [0.072s] Masking low complexity seeds... [0.016s] Searching alignments... [0.23s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 4/4. Building reference seed array... [0.131s] Building query seed array... [0.11s] Computing hash join... [0.071s] Masking low complexity seeds... [0.018s] Searching alignments... [0.239s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 1/4. Building reference seed array... [0.13s] Building query seed array... [0.111s] Computing hash join... [0.074s] Masking low complexity seeds... [0.017s] Searching alignments... [0.228s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 2/4. Building reference seed array... [0.155s] Building query seed array... [0.124s] Computing hash join... [0.075s] Masking low complexity seeds... [0.016s] Searching alignments... [0.226s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 3/4. Building reference seed array... [0.163s] Building query seed array... [0.125s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.224s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 4/4. Building reference seed array... [0.132s] Building query seed array... [0.11s] Computing hash join... [0.072s] Masking low complexity seeds... [0.019s] Searching alignments... [0.225s] Deallocating memory... [0s] Loading trace points... [0.178s] Sorting trace points... [0.032s] Computing alignments... [7.923s] Deallocating buffers... [0.012s] Loading trace points... [0s] Loading reference sequences... [0s] Deallocating buffers... [0s] Deallocating queries... [0.019s] Loading query sequences... [0s] Closing the input file... [0s] Closing the output file... [0s] Closing the database... [0.002s] Cleaning up... [0s] Total time = 53.293s Reported 9141 pairwise alignments, 9141 HSPs. 9141 queries aligned.