Bismark report for: ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-178-TP2_S1_R1_001.fastq.gz and ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-178-TP2_S1_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/D-Apul/data/ with the specified options: -q --score-min L,0,-1.0 -p 48 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 182967324 Number of paired-end alignments with a unique best hit: 94962955 Mapping efficiency: 51.9% Sequence pairs with no alignments under any condition: 47926569 Sequence pairs did not map uniquely: 40077800 Sequence pairs which were discarded because genomic sequence could not be extracted: 154 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 27514924 ((converted) top strand) GA/CT/CT: 19970680 (complementary to (converted) top strand) GA/CT/GA: 19958240 (complementary to (converted) bottom strand) CT/GA/GA: 27518957 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3994751719 Total methylated C's in CpG context: 70399594 Total methylated C's in CHG context: 12957412 Total methylated C's in CHH context: 62458470 Total methylated C's in Unknown context: 11680962 Total unmethylated C's in CpG context: 629501595 Total unmethylated C's in CHG context: 739934104 Total unmethylated C's in CHH context: 2479500544 Total unmethylated C's in Unknown context: 43368441 C methylated in CpG context: 10.1% C methylated in CHG context: 1.7% C methylated in CHH context: 2.5% C methylated in unknown context (CN or CHN): 21.2% Bismark completed in 1d 1h 6m 22s