Bismark report for: ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-173-TP2_S4_R1_001.fastq.gz and ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-173-TP2_S4_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/D-Apul/data/ with the specified options: -q --score-min L,0,-1.0 -p 48 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 213856184 Number of paired-end alignments with a unique best hit: 108437982 Mapping efficiency: 50.7% Sequence pairs with no alignments under any condition: 60420323 Sequence pairs did not map uniquely: 44997879 Sequence pairs which were discarded because genomic sequence could not be extracted: 149 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 31883243 ((converted) top strand) GA/CT/CT: 22449007 (complementary to (converted) top strand) GA/CT/GA: 22363582 (complementary to (converted) bottom strand) CT/GA/GA: 31742001 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4684212694 Total methylated C's in CpG context: 76855937 Total methylated C's in CHG context: 14470774 Total methylated C's in CHH context: 69704529 Total methylated C's in Unknown context: 12705187 Total unmethylated C's in CpG context: 734021072 Total unmethylated C's in CHG context: 855050390 Total unmethylated C's in CHH context: 2934109992 Total unmethylated C's in Unknown context: 48870104 C methylated in CpG context: 9.5% C methylated in CHG context: 1.7% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 20.6% Bismark completed in 1d 8h 35m 35s