Bismark report for: ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-150-TP2_S2_R1_001.fastq.gz and ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-150-TP2_S2_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/D-Apul/data/ with the specified options: -q --score-min L,0,-1.0 -p 48 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 234083660 Number of paired-end alignments with a unique best hit: 113214924 Mapping efficiency: 48.4% Sequence pairs with no alignments under any condition: 73776618 Sequence pairs did not map uniquely: 47092118 Sequence pairs which were discarded because genomic sequence could not be extracted: 117 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 35012218 ((converted) top strand) GA/CT/CT: 21625620 (complementary to (converted) top strand) GA/CT/GA: 21552127 (complementary to (converted) bottom strand) CT/GA/GA: 35024842 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4826708375 Total methylated C's in CpG context: 89607039 Total methylated C's in CHG context: 18299207 Total methylated C's in CHH context: 86677563 Total methylated C's in Unknown context: 13932902 Total unmethylated C's in CpG context: 742472247 Total unmethylated C's in CHG context: 882032802 Total unmethylated C's in CHH context: 3007619517 Total unmethylated C's in Unknown context: 48070600 C methylated in CpG context: 10.8% C methylated in CHG context: 2.0% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 22.5% Bismark completed in 1d 23h 45m 46s