Bismark report for: ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-145-TP2_S3_R1_001.fastq.gz and ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-145-TP2_S3_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/D-Apul/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 244866108 Number of paired-end alignments with a unique best hit: 128317307 Mapping efficiency: 52.4% Sequence pairs with no alignments under any condition: 64999585 Sequence pairs did not map uniquely: 51549216 Sequence pairs which were discarded because genomic sequence could not be extracted: 139 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 38248879 ((converted) top strand) GA/CT/CT: 26115718 (complementary to (converted) top strand) GA/CT/GA: 25906262 (complementary to (converted) bottom strand) CT/GA/GA: 38046309 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5649675342 Total methylated C's in CpG context: 85511979 Total methylated C's in CHG context: 18977692 Total methylated C's in CHH context: 88350399 Total methylated C's in Unknown context: 16139487 Total unmethylated C's in CpG context: 878134725 Total unmethylated C's in CHG context: 1019570879 Total unmethylated C's in CHH context: 3559129668 Total unmethylated C's in Unknown context: 58014953 C methylated in CpG context: 8.9% C methylated in CHG context: 1.8% C methylated in CHH context: 2.4% C methylated in unknown context (CN or CHN): 21.8% Bismark completed in 1d 11h 50m 4s