Bismark report for: ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-140-TP2_S5_R1_001.fastq.gz and ../output/01.00-D-Apul-WGBS-trimming-cutadapt-FastQC-MultiQC/trimmed_ACR-140-TP2_S5_R2_001.fastq.gz (version: v0.23.1) Bismark was run with Bowtie 2 against the bisulfite genome of /scratch3/workspace/zdellaert_uri_edu-deep_dive/deep-dive-expression/D-Apul/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 253975571 Number of paired-end alignments with a unique best hit: 129549817 Mapping efficiency: 51.0% Sequence pairs with no alignments under any condition: 74033883 Sequence pairs did not map uniquely: 50391871 Sequence pairs which were discarded because genomic sequence could not be extracted: 223 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 38859209 ((converted) top strand) GA/CT/CT: 25950331 (complementary to (converted) top strand) GA/CT/GA: 25911923 (complementary to (converted) bottom strand) CT/GA/GA: 38828131 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5587802285 Total methylated C's in CpG context: 98818956 Total methylated C's in CHG context: 18036512 Total methylated C's in CHH context: 87165516 Total methylated C's in Unknown context: 16505398 Total unmethylated C's in CpG context: 857492373 Total unmethylated C's in CHG context: 1013978029 Total unmethylated C's in CHH context: 3512310899 Total unmethylated C's in Unknown context: 62100765 C methylated in CpG context: 10.3% C methylated in CHG context: 1.7% C methylated in CHH context: 2.4% C methylated in unknown context (CN or CHN): 21.0% Bismark completed in 1d 9h 59m 58s